LNA-5-Me-U CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C41H49N4O9P
- MW
- 772.8
- Purity
- 99.3% (NMR)
LNA-5-Me-U CE Phosphoramidite is the locked nucleic acid (LNA) 5-methyluridine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) 5-methyluridine (5-methyluracil base) with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65048 is identified by CAS 206055-75-6, molecular formula C41H49N4O9P, molecular weight 772.8, PubChem CID 137628654, and InChIKey STPXOEPMLLHUDQ-DMMPMGKKSA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required.
Synonyms
DMT-LNA-5Me-U phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-5-methyluridine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65048 |
| CAS No. | 206055-75-6 |
| Molecular Formula | C41H49N4O9P |
| Molecular Weight | 772.8 |
| PubChem CID | 137628654 |
| InChIKey | STPXOEPMLLHUDQ-DMMPMGKKSA-N |
| Purity | 99.3% (NMR) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- 5-Methyluracil
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- None required
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Application context
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- No base protection needed: the 5-methyluracil base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 137628654
PubChem · CID 137628654
Frequently Asked Questions
Why does this LNA amidite have no base protecting group?
Its base is 5-methyluracil, which has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
What is LNA?
LNA (locked nucleic acid) contains a rigid 2'-O,4'-C-methylene bridge. In this product, that locked sugar is part of a 5-methyluridine phosphoramidite building block for oligonucleotide synthesis.
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