5'-O-DMT-N4-isobutyryl-2'-deoxycytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Resumen del producto
Vista rápida
- Familia de productos
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C34H37N3O7
- PM
- 599.68
- Pureza
- 99.63%
5'-O-DMT-N4-isobutyryl-2'-deoxycytidine is 2'-deoxycytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-isobutyryl base-protecting group. CH64109 is identified by CAS 100898-62-2, molecular formula C34H37N3O7, molecular weight 599.68, PubChem CID 13888004, and InChIKey UGBSAMWXUKFNAH-ILJQZKEFSA-N.
It is a doubly protected precursor for DNA phosphoramidite/oligonucleotide synthesis: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, while the N4-isobutyryl group masks the cytosine exocyclic amine and is removed at final deprotection.
Sinónimos
5'-O-DMT-N4-isobutyryl-2'-deoxycytidine; 5'-O-(4,4'-dimethoxytrityl)-N4-isobutyryl-dC; DMT-dCiBu
Identidad y especificaciones
| Catalog No. | CH64109 |
| CAS No. | 100898-62-2 |
| Molecular Formula | C34H37N3O7 |
| Molecular Weight | 599.68 |
| PubChem CID | 13888004 |
| InChIKey | UGBSAMWXUKFNAH-ILJQZKEFSA-N |
| Purity | 99.63% |
Condiciones de almacenamiento
0-8 C
Atributos técnicos
- Nucleobase
- Cytosine
- Sugar
- 2'-Deoxyribose
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- N4-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 50 mg; 100 mg; 1 g; 5 g; 25 g; 100 g; 250 g; 1 kg; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white, off-white to faint yellow powder
Contexto de aplicación
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each oligonucleotide chain-elongation cycle.
- N4-isobutyryl base protection: the isobutyryl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
- Phosphoramidite precursor context: a doubly protected 2'-deoxycytidine precursor toward DNA phosphoramidite building blocks.
Recursos técnicos relacionados
Fuentes públicas
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Compuesto de PubChem 13888004
PubChem · CID 13888004
Preguntas frecuentes
What do the two protecting groups on this deoxycytidine do?
The 5'-O-DMT (dimethoxytrityl) group is an acid-labile protection on the 5'-hydroxyl that is removed at the start of each oligonucleotide chain-elongation cycle; the N4-isobutyryl group protects the cytosine exocyclic amine during synthesis and is removed at final deprotection.
How does it differ from 5'-O-DMT-N4-benzoyl-2'-deoxycytidine?
Both are 5'-O-DMT-protected 2'-deoxycytidines with a protected cytosine N4-amine, but this one uses an isobutyryl group rather than benzoyl. The benzoyl and acetyl forms are cataloged separately.
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