2'-Deoxycytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Resumen del producto
Vista rápida
- Familia de productos
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C46H52N5O8P
- PM
- 833.9
- Pureza
- 100% (NMR)
2'-Deoxycytidine (Bz) CE Phosphoramidite is the fully protected 2'-deoxycytidine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65002 is identified by CAS 102212-98-6, molecular formula C46H52N5O8P, molecular weight 833.9, PubChem CID 9832450, and InChIKey PGTNFMKLGRFZDX-SALLYJDFSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, and the N4-benzoyl base group is removed at final deprotection.
Sinónimos
DMT-dC(Bz) phosphoramidite; N4-benzoyl-5'-O-DMT-2'-deoxycytidine 3'-CE phosphoramidite
Identidad y especificaciones
| Catalog No. | CH65002 |
| CAS No. | 102212-98-6 |
| Molecular Formula | C46H52N5O8P |
| Molecular Weight | 833.9 |
| PubChem CID | 9832450 |
| InChIKey | PGTNFMKLGRFZDX-SALLYJDFSA-N |
| Purity | 100% (NMR) |
Condiciones de almacenamiento
-20 C
Atributos técnicos
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Cytosine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- N4-Benzoyl
- Grade
- TheraPure; Standard grade
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; 1 kg; Custom packaging on request
- Water content
- <=0.3%
- Coupling efficiency
- spec >=98.0%; observed 98.0%
- Physical form
- white to pale yellow powder
- Stability
- moisture sensitive; heat sensitive
Contexto de aplicación
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- N4-benzoyl base protection: the benzoyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
Recursos técnicos relacionados
Fuentes públicas
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Compuesto de PubChem 9832450
PubChem · CID 9832450
Preguntas frecuentes
What makes this a phosphoramidite building block?
It carries a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group — the reactive coupling group used in automated solid-phase DNA synthesis. It also has an acid-labile 5'-O-DMT group (removed each cycle) and an N4-benzoyl base protection (removed at final deprotection).
How does it relate to 5'-O-DMT-N4-benzoyl-2'-deoxycytidine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The 5'-O-DMT-N4-benzoyl-2'-deoxycytidine precursor is cataloged separately.
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