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Oligonucleotide Synthesis, RNA Raw Materials & Modification

2'-OMe-Cytidine (Bz) CE Phosphoramidite

Oligonucleotide Synthesis, RNA Raw Materials & Modification

N.º de productoCH65023N.º CAS110764-78-8Pureza>99.00%
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Resumen del producto

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Familia de productos
Oligonucleotide Synthesis, RNA Raw Materials & Modification
FM
C47H54N5O9P
PM
863.9
Pureza
>99.00%

2'-OMe-Cytidine (Bz) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65023 is identified by CAS 110764-78-8, molecular formula C47H54N5O9P, molecular weight 863.9, PubChem CID 13872225, and InChIKey JFFSFQRVIPPCBC-VKBHKTMGSA-N.

Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.

Sinónimos

DMT-2'-OMe-rC(Bz) phosphoramidite; 5'-O-DMT-N4-benzoyl-2'-O-methylcytidine 3'-CE phosphoramidite

Identidad y especificaciones

Catalog No.CH65023
CAS No.110764-78-8
Molecular FormulaC47H54N5O9P
Molecular Weight863.9
PubChem CID13872225
InChIKeyJFFSFQRVIPPCBC-VKBHKTMGSA-N
Purity>99.00%

Condiciones de almacenamiento

-20 C

Atributos técnicos

Monomer class
2'-O-methyl RNA CE phosphoramidite
Nucleobase
Cytosine
Sugar modification
2'-O-Methyl
Base protection
N4-Benzoyl
Grade
N (Normal)
Packaging
100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 25 g; 100 g; Custom packaging on request
Physical form
white powder
Stability
>=4 years

Contexto de aplicación

  • 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
  • 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
  • 5'-DMT + N4-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection.

Recursos técnicos relacionados

Fuentes públicas

Preguntas frecuentes

What does this 2'-OMe amidite do in RNA synthesis?

Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N4-benzoyl removed at final deprotection. The product provides a 2'-O-methylcytidine phosphoramidite identity.

How does it differ from the 2'-OMe-C(Ac) phosphoramidite?

Both are 2'-O-methyl cytidine amidites, but this one uses an N4-benzoyl base protection rather than acetyl. The acetyl amidite is cataloged separately.

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