2'-F-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Resumen del producto
Vista rápida
- Familia de productos
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C44H53FN7O8P
- PM
- 857.9
- Pureza
- 99.9% (HPLC)
2'-F-Guanosine (iBu) CE Phosphoramidite is the 2'-fluoro guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65028 is identified by CAS 144089-97-4, molecular formula C44H53FN7O8P, molecular weight 857.9, PubChem CID 135448972, and InChIKey KJFUMXZZVYMQEU-AOCJBPQJSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
Sinónimos
DMT-2'-F-rG(iBu) phosphoramidite; 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine 3'-CE phosphoramidite
Identidad y especificaciones
| Catalog No. | CH65028 |
| CAS No. | 144089-97-4 |
| Molecular Formula | C44H53FN7O8P |
| Molecular Weight | 857.9 |
| PubChem CID | 135448972 |
| InChIKey | KJFUMXZZVYMQEU-AOCJBPQJSA-N |
| Purity | 99.9% (HPLC) |
Condiciones de almacenamiento
-20 C
Atributos técnicos
- Monomer class
- 2'-fluoro RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N2-Isobutyryl
- Packaging
- 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.3%
- Physical form
- white to off-white powder
- Stability
- stable under recommended storage conditions
Contexto de aplicación
- 2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Recursos técnicos relacionados
Fuentes públicas
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Compuesto de PubChem 135448972
PubChem · CID 135448972
Preguntas frecuentes
What does this 2'-F amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-isobutyryl removed at final deprotection. The product provides a 2'-fluoro guanosine phosphoramidite identity.
How does it relate to 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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