3'-O-Ac-2'-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Resumen del producto
Vista rápida
- Familia de productos
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- FM
- C12H19N2O15P3
- PM
- 524.20
- Pureza
- >95% (HPLC)
3'-O-Ac-2'-dTTP is 3'-O-acetyl-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. Key identifiers include CAS 90290-82-7, molecular formula C12H19N2O15P3, and molecular weight 524.20 (PubChem CID 168301121).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-acetyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Sinónimos
3'-O-acetyl-dTTP; 3'-O-(acetyl)-thymidine-5'-triphosphate
Identidad y especificaciones
| Catalog No. | CH66033 |
| CAS No. | 90290-82-7 |
| Molecular Formula | C12H19N2O15P3 |
| Molecular Weight | 524.20 |
| PubChem CID | 168301121 |
| InChIKey | PEHYLTBXIMDUCN-IVZWLZJFSA-N |
| Purity | >95% (HPLC) |
Condiciones de almacenamiento
-70 C
Atributos técnicos
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Acetyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 10 mg; 50 mg; 100 mg; 250 mg; 5 umol (~2.6 mg) in 500 uL vial; Custom packaging on request
- Solution concentration
- 10 mM
- Physical form
- 10 mM aqueous sodium-salt solution
- Stability
- slowly decomposes at ambient temperature; thaw only before use and avoid repeated freeze/thaw cycles
Contexto de aplicación
- Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-acetyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Thymine building block: supplies the thymine reversible terminator, completing the four-nucleotide (A/C/G/T) 3'-O-acetyl SBS set.
Recursos técnicos relacionados
Fuentes públicas
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- Compuesto de PubChem 168301121
PubChem · CID 168301121
Preguntas frecuentes
What is a 3'-O-acetyl reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the acetyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the thymine (T) reversible terminator — 3'-O-acetyl-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-acetyl terminators it forms a four-nucleotide set for sequencing-by-synthesis.
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