Thymidine (2'-Deoxythymidine)
1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Chemical Name
- 1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
- MF
- C10H14N2O5
- MW
- 242.23
- Purity
- 99.9% (HPLC)
Thymidine, also written 2'-deoxythymidine (dT), is the thymine DNA nucleoside formed from thymine and 2-deoxy-β-D-ribose. CH64004 is identified by CAS 50-89-5, molecular formula C10H14N2O5, molecular weight 242.23, and PubChem CID 5789.
The source label "2'-deoxythymine" should be read as thymidine / 2'-deoxythymidine, not as the free base thymine. Thymidine is used in thymidine-kinase phosphorylation research and is the parent nucleoside behind tritiated-thymidine DNA-synthesis assays.
Synonyms
Thymidine; 2'-deoxythymidine; dT; deoxythymidine; thymine 2'-deoxyriboside
Identity and specifications
| Catalog No. | CH64004 |
| CAS No. | 50-89-5 |
| Molecular Formula | C10H14N2O5 |
| Molecular Weight | 242.23 |
| PubChem CID | 5789 |
| InChIKey | IQFYYKKMVGJFEH-XLPZGREQSA-N |
| Purity | 99.9% (HPLC) |
Technical attributes
- Nucleobase
- Thymine (5-methyluracil)
- Sugar
- 2'-Deoxyribose
- Nucleoside class
- Pyrimidine 2'-deoxyribonucleoside
- Packaging
- Custom packaging on request
- Water content
- <=0.5% KF
- Physical form
- White to almost white crystalline powder
- Stability
- Released 2024-05-13; retest May 2029
Application context
- Thymidine-kinase research: literature reports thymidine as a substrate of thymidine kinase in phosphorylation studies.
- DNA-synthesis assays: tritiated thymidine ([3H]thymidine) is a classic DNA-synthesis marker, with thymidine as the parent nucleoside.
- Thymine DNA nucleoside reference: CH64004 provides the dT identity for nucleoside and oligonucleotide chemistry comparisons.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 5789
PubChem · CID 5789
Frequently Asked Questions
Is CH64004 thymidine or the base thymine?
It is thymidine, the nucleoside (CAS 50-89-5) — thymine joined to 2-deoxyribose. The brochure label "2'-deoxythymine" is imprecise; the free base thymine is a separate compound.
Why is thymidine used in DNA-synthesis assays?
Tritiated thymidine ([3H]thymidine) is incorporated into newly synthesized DNA, making it a classic reference marker for DNA synthesis; thymidine is the parent nucleoside for these assays.
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