5-Methyluridine (Ribothymidine)
1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Chemical Name
- 1-[(2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
- MF
- C10H14N2O6
- MW
- 258.23
- Purity
- >98.0% (HPLC, titration)
5-Methyluridine, also known as ribothymidine (rT), is a modified pyrimidine RNA nucleoside with 5-methyluracil linked to β-D-ribose. CH64014 is identified by CAS 1463-10-1, molecular formula C10H14N2O6, molecular weight 258.23, and PubChem CID 445408. It is distinct from thymidine (CH64004), which contains 2-deoxyribose.
In tRNA literature, ribothymidine is the conserved modified nucleoside at position 54 of the TΨC loop. This supports its use as a reference residue in tRNA-modification and modified-RNA chemistry research.
Synonyms
5-Methyluridine; ribothymidine; rT; 5-methyluracil riboside; m5U
Identity and specifications
| Catalog No. | CH64014 |
| CAS No. | 1463-10-1 |
| Molecular Formula | C10H14N2O6 |
| Molecular Weight | 258.23 |
| PubChem CID | 445408 |
| InChIKey | DWRXFEITVBNRMK-JXOAFFINSA-N |
| Purity | >98.0% (HPLC, titration) |
Storage conditions
Room temperature; cool and dark place below 15 C; inert gas
Technical attributes
- Nucleobase
- 5-Methyluracil
- Sugar
- D-Ribose
- Base modification
- 5-Methyl
- Nucleoside class
- Modified pyrimidine ribonucleoside
- Packaging
- 1 g glass bottle with plastic insert; Custom packaging on request
- Water content
- <=5.0%
- Physical form
- Solid; white to almost white powder to crystal
Application context
- tRNA-modification reference: 5-methyluridine is ribothymidine (rT), the conserved modified nucleoside at position 54 of the tRNA TΨC loop.
- Modified RNA nucleoside reference: CH64014 provides the 5-methyluridine / ribothymidine identity for modified-RNA and nucleoside chemistry comparisons.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 445408
PubChem · CID 445408
Frequently Asked Questions
What is the difference between 5-methyluridine and thymidine?
5-Methyluridine (ribothymidine) is the RNA nucleoside — 5-methyluracil on ribose. Thymidine is the DNA nucleoside — the same base on 2-deoxyribose. They are cataloged separately.
Why is 5-methyluridine relevant to tRNA research?
As ribothymidine (rT), it is the conserved modified nucleoside at position 54 of the tRNA TΨC loop, so it is a reference residue in studies of tRNA modification.
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