2',3'-Dideoxythymidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H14N2O4
- MW
- 226.23
- Purity
- >98.0% (HPLC, titration)
2',3'-Dideoxythymidine (ddT) is a pyrimidine 2',3'-dideoxynucleoside with thymine attached to a sugar lacking both the 2'- and 3'-hydroxyl groups. CH64018 is identified by CAS 3416-05-5, molecular formula C10H14N2O4, molecular weight 226.23, and PubChem CID 65119. It is distinct from the didehydro analog d4T.
After conversion to the corresponding triphosphate, the missing 3'-hydroxyl prevents further DNA-chain extension after polymerase incorporation. That property defines its chain-termination context in DNA-polymerase and dideoxy-sequencing research.
Synonyms
2',3'-Dideoxythymidine; ddT; 3'-deoxythymidine analog (see Identity Check)
Identity and specifications
| Catalog No. | CH64018 |
| CAS No. | 3416-05-5 |
| Molecular Formula | C10H14N2O4 |
| Molecular Weight | 226.23 |
| PubChem CID | 65119 |
| InChIKey | XKKCQTLDIPIRQD-JGVFFNPUSA-N |
| Purity | >98.0% (HPLC, titration) |
Storage conditions
Room temperature; cool and dark place below 15 C; inert gas
Technical attributes
- Nucleobase
- Thymine (5-methyluracil)
- Sugar
- 2',3'-Dideoxyribose
- Nucleoside class
- Pyrimidine dideoxynucleoside
- Research role
- Chain-terminator nucleotide precursor
- Packaging
- 250 mg glass bottle with plastic insert; Custom packaging on request
- Physical form
- Solid; white to light yellow powder to crystal
- Stability
- >=4 years
Application context
- Chain-terminator precursor: the corresponding ddTTP is incorporated by DNA polymerase as a chain-terminating nucleotide in dideoxy-sequencing research.
- DNA-polymerase research: ddT provides the thymidine dideoxynucleoside identity for polymerase incorporation and chain-termination studies.
- Dideoxynucleoside reference: CH64018 provides the 2',3'-dideoxythymidine identity for nucleoside chemistry comparisons.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 65119
PubChem · CID 65119
Frequently Asked Questions
Why is 2',3'-dideoxythymidine a chain terminator?
It lacks a 3'-hydroxyl, so once its triphosphate is incorporated by DNA polymerase no further nucleotide can be added and the chain terminates — the principle behind dideoxy sequencing.
Is ddT the same as the didehydro d4T analog?
No. CH64018 is 2',3'-dideoxythymidine; d4T is 2',3'-didehydro-2',3'-dideoxythymidine, a different compound with an added double bond.
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