N4-Isobutyryl-2'-deoxycytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C13H19N3O5
- MW
- 297.31
- Purity
- >=99%
N4-Isobutyryl-2'-deoxycytidine (dCiBu) is 2'-deoxycytidine whose cytosine exocyclic N4-amine is protected with an isobutyryl group. CH64077 is identified by CAS 110522-75-3, molecular formula C13H19N3O5, molecular weight 297.31, PubChem CID 688503, and InChIKey PDTIVAGPIHEWDX-IQJOONFLSA-N.
The cited oligonucleotide-synthesis literature discusses isobutyryl as a labile cytosine base-protecting group and describes removal of base-protecting groups during deprotection. CH64077 provides the N4-isobutyryl-protected 2'-deoxycytidine identity for DNA building-block and nucleoside chemistry research.
Synonyms
N4-Isobutyryl-2'-deoxycytidine; N4-ibu-dC; dCiBu
Identity and specifications
| Catalog No. | CH64077 |
| CAS No. | 110522-75-3 |
| Molecular Formula | C13H19N3O5 |
| Molecular Weight | 297.31 |
| PubChem CID | 688503 |
| InChIKey | PDTIVAGPIHEWDX-IQJOONFLSA-N |
| Purity | >=99% |
Storage conditions
0-8 C
Technical attributes
- Nucleobase
- Cytosine
- Sugar
- 2'-Deoxyribose
- Base protection
- N4-Isobutyryl
- Synthesis role
- Base-protected DNA nucleoside intermediate
- Packaging
- 1 g; 5 g; 10 g; 25 g; Custom packaging on request
- Physical form
- white powder
Application context
- Base-protected DNA building block: the N4-isobutyryl group masks the cytosine exocyclic amine in oligonucleotide-synthesis chemistry and is removed during deprotection.
- Cytosine (dC) monomer precursor context: CH64077 is the isobutyryl-protected 2'-deoxycytidine identity relevant to cytosine building-block preparation.
- Nucleoside chemistry reference: CH64077 provides the N4-isobutyryl-2'-deoxycytidine (dCiBu) identity for nucleoside chemistry research.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 688503
PubChem · CID 688503
Frequently Asked Questions
Why is the N4-amine of this deoxycytidine protected with isobutyryl?
In oligonucleotide synthesis the reactive exocyclic amine of cytosine is masked with a labile protecting group such as isobutyryl (a fast-deprotection cytosine protection); the group is later removed during deprotection.
How does it differ from N4-benzoyl- or N4-acetyl-2'-deoxycytidine?
All three protect the cytosine N4-amine, but with different acyl groups (isobutyryl, benzoyl, or acetyl). The benzoyl and acetyl forms are cataloged separately.
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