N4-Acetyl-2'-O-methylcytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C12H17N3O6
- MW
- 299.28
- Purity
- 99.62%
N4-Acetyl-2'-O-methylcytidine is 2'-O-methylcytidine whose cytosine exocyclic N4-amine is protected with an acetyl group. CH64090 is identified by CAS 113886-71-8, molecular formula C12H17N3O6, molecular weight 299.28, PubChem CID 10902616, and InChIKey CYDFBLGNJUNSCC-QCNRFFRDSA-N.
The cited oligonucleotide-synthesis literature discusses exocyclic-amine protecting groups and their removal during deprotection. In the cited 2'-O-methyl literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
N4-Acetyl-2'-O-methylcytidine; N4-Ac-2'-OMe-rC; 2'-OMe-rC(Ac)
Identity and specifications
| Catalog No. | CH64090 |
| CAS No. | 113886-71-8 |
| Molecular Formula | C12H17N3O6 |
| Molecular Weight | 299.28 |
| PubChem CID | 10902616 |
| InChIKey | CYDFBLGNJUNSCC-QCNRFFRDSA-N |
| Purity | 99.62% |
Storage conditions
+4 C
Technical attributes
- Nucleobase
- Cytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Acetyl
- Synthesis role
- Base-protected 2'-O-methyl nucleoside intermediate
- Packaging
- 10 mg; 25 mg; 100 mg; 500 mg; 1 mL x 10 mM (in DMSO); Custom packaging on request
- Solution concentration
- 10 mM in DMSO
- Water content
- <=2.0%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Application context
- Base-protected 2'-OMe building block: the N4-acetyl group masks the cytosine exocyclic amine in oligonucleotide-synthesis chemistry and is removed during deprotection.
- 2'-OMe sugar modification context: cited 2'-O-methyl literature discusses 2'-OMe residues for nuclease resistance and RNA affinity.
- Nucleoside chemistry reference: CH64090 provides the N4-acetyl-2'-O-methylcytidine identity for nucleoside chemistry research.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 10902616
PubChem · CID 10902616
Frequently Asked Questions
How does it differ from N4-benzoyl-2'-O-methylcytidine?
Both are 2'-O-methylcytidine with a protected cytosine N4-amine, but this one uses an acetyl group rather than benzoyl. The benzoyl form is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.