2'-O-MOE-N4-Benzoyl-5-methylcytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C20H25N3O7
- MW
- 419.43
- Purity
- >=98%
2'-O-MOE-N4-Benzoyl-5-methylcytidine is 2'-O-(2-methoxyethyl)-5-methylcytidine whose cytosine exocyclic N4-amine is protected with a benzoyl group; its base is 5-methylcytosine. CH64097 is identified by CAS 340162-93-8, molecular formula C20H25N3O7, molecular weight 419.43, PubChem CID 20660421, and InChIKey LGGHYGUGIGYDFX-YKTARERQSA-N.
The cited oligonucleotide-synthesis literature discusses exocyclic-amine protecting groups and their removal during deprotection. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
2'-O-MOE-N4-Benzoyl-5-methylcytidine; 2'-O-(2-methoxyethyl)-N4-benzoyl-5-methylcytidine; MOE-5Me-rC(Bz)
Identity and specifications
| Catalog No. | CH64097 |
| CAS No. | 340162-93-8 |
| Molecular Formula | C20H25N3O7 |
| Molecular Weight | 419.43 |
| PubChem CID | 20660421 |
| InChIKey | LGGHYGUGIGYDFX-YKTARERQSA-N |
| Purity | >=98% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- 5-Methylcytosine
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- N4-Benzoyl
- Synthesis role
- Base-protected MOE nucleoside intermediate
- Packaging
- 5 mg; 10 mg; 25 mg; 50 mg; 100 mg; 500 mg; 10 g; 100 g; 1 kg; Custom packaging on request
- Physical form
- white to off-white powder
- Stability
- Powder: 3 years at -20 C; in solvent: 1 year at -80 C
Application context
- Base-protected 2'-O-MOE building block: the N4-benzoyl group masks the cytosine exocyclic amine in oligonucleotide-synthesis chemistry and is removed during deprotection.
- 2'-O-MOE modification context: cited structure and stability literature discusses 2'-O-MOE residues in relation to RNA affinity, nuclease resistance, and gapmer flanking residues.
- 5-Methylcytosine base: the compound contains a 5-methylcytosine base, described here as a structural identity feature.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 20660421
PubChem · CID 20660421
Frequently Asked Questions
How does it differ from 2'-O-MOE-N4-benzoylcytidine?
Both are N4-benzoyl base-protected 2'-MOE cytidines, but this one has a 5-methylcytosine base (an extra methyl on the base). The non-methylated form is cataloged separately.
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