5'-O-DMT-thymidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C31H32N2O7
- MW
- 544.60
- Purity
- >=98.5% (HPLC)
5'-O-DMT-thymidine is thymidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group. CH64102 is identified by CAS 40615-39-2, molecular formula C31H32N2O7, molecular weight 544.60, PubChem CID 162419, and InChIKey UBTJZUKVKGZHAD-UPRLRBBYSA-N.
It is a 5'-protected precursor for DNA phosphoramidite/oligonucleotide synthesis: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle. Because thymine has no exocyclic amine, no base protecting group is required.
Synonyms
5'-O-DMT-thymidine; 5'-O-(4,4'-dimethoxytrityl)thymidine; DMT-dT
Identity and specifications
| Catalog No. | CH64102 |
| CAS No. | 40615-39-2 |
| Molecular Formula | C31H32N2O7 |
| Molecular Weight | 544.60 |
| PubChem CID | 162419 |
| InChIKey | UBTJZUKVKGZHAD-UPRLRBBYSA-N |
| Purity | >=98.5% (HPLC) |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- Thymine (5-methyluracil)
- Sugar
- 2'-Deoxyribose
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 5 g; 25 g; 100 g; 250 g; 500 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder to crystalline powder
- Stability
- >=4 years
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each oligonucleotide chain-elongation cycle.
- No base protection needed: thymine has no exocyclic amine, so this building block requires no base protecting group.
- Phosphoramidite precursor context: a 5'-protected thymidine precursor toward DNA phosphoramidite building blocks.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 162419
PubChem · CID 162419
Frequently Asked Questions
Why does 5'-O-DMT-thymidine have no base protecting group?
Thymine has no reactive exocyclic amine, so unlike A, C, and G it needs no base protection. Only the 5'-hydroxyl is protected with the acid-labile DMT group, which is removed at the start of each oligonucleotide chain-elongation cycle.
How does it differ from thymidine?
It carries a 5'-O-(4,4'-dimethoxytrityl) group on the 5'-hydroxyl, whereas thymidine is unprotected. Thymidine is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.