5'-O-DMT-2'-O-TBDMS-N6-methyladenosine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C38H47N5O6Si
- MW
- 697.9
- Purity
- >=98.5%
5'-O-DMT-2'-O-TBDMS-N6-methyladenosine is N6-methyladenosine (m6A) carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and a 2'-O-(tert-butyldimethylsilyl) (TBDMS) group. CH64114 is identified by CAS 588698-75-3, molecular formula C38H47N5O6Si, molecular weight 697.9, PubChem CID 12994119, and InChIKey KEEBYCOQGORZDK-QGGAYTEESA-N.
It is an RNA phosphoramidite precursor context for m6A-modified RNA synthesis research: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during RNA synthesis. The N6-methyl group is the permanent m6A base modification, not a removable protecting group.
Synonyms
5'-O-DMT-2'-O-TBDMS-N6-methyladenosine; 5'-O-DMT-2'-O-TBDMS-m6A; DMT-2'-TBDMS-m6A
Identity and specifications
| Catalog No. | CH64114 |
| CAS No. | 588698-75-3 |
| Molecular Formula | C38H47N5O6Si |
| Molecular Weight | 697.9 |
| PubChem CID | 12994119 |
| InChIKey | KEEBYCOQGORZDK-QGGAYTEESA-N |
| Purity | >=98.5% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- Adenine
- 5'-O protection
- DMT (dimethoxytrityl)
- 2'-O protection
- TBDMS (tert-butyldimethylsilyl)
- Base modification
- N6-Methyl (m6A)
- Packaging
- 100 mg; 250 mg; 1 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
Application context
- m6A-modified RNA synthesis research: the base is N6-methyladenosine (m6A), treated here as a permanent base modification rather than a protecting group.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-O-TBDMS 2'-OH protection: the tert-butyldimethylsilyl group protects the ribose 2'-hydroxyl during solid-phase RNA synthesis.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 12994119
PubChem · CID 12994119
Frequently Asked Questions
Is the N6-methyl group a protecting group?
No. The N6-methyl is the permanent m6A base modification. Only the 5'-hydroxyl (acid-labile DMT, removed each cycle) and the ribose 2'-hydroxyl (TBDMS, for RNA synthesis) carry protecting groups.
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