5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C36H39N5O8
- MW
- 669.7
- Purity
- 99%
5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine is 2'-O-methylguanosine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N2-isobutyryl base-protecting group. CH64118 is identified by CAS 114745-26-5, molecular formula C36H39N5O8, molecular weight 669.7, PubChem CID 135742507, and InChIKey ISQLJOGRNUQHJX-WIFIACMTSA-N.
It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N2-isobutyryl group masks the guanine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine; 5'-O-DMT-2'-OMe-rG(iBu); DMT-2'-OMe-rG(iBu)
Identity and specifications
| Catalog No. | CH64118 |
| CAS No. | 114745-26-5 |
| Molecular Formula | C36H39N5O8 |
| Molecular Weight | 669.7 |
| PubChem CID | 135742507 |
| InChIKey | ISQLJOGRNUQHJX-WIFIACMTSA-N |
| Purity | 99% |
Storage conditions
-20 C
Technical attributes
- Nucleobase
- Guanine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 25 mg; 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
- Stability
- 3 years at -20 C; 2 years at 4 C
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-OMe modification context: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135742507
PubChem · CID 135742507
Frequently Asked Questions
How does it differ from N2-isobutyryl-2'-O-methylguanosine?
Both carry an N2-isobutyryl-protected 2'-O-methylguanosine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.