5'-O-DMT-N4-acetyl-2'-O-methylcytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C33H35N3O8
- MW
- 601.6
- Purity
- >=99%
5'-O-DMT-N4-acetyl-2'-O-methylcytidine is 2'-O-methylcytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-acetyl base-protecting group. CH64121 is identified by CAS 199593-08-3, molecular formula C33H35N3O8, molecular weight 601.6, PubChem CID 70700566, and InChIKey FINUHOJILDNELQ-PMFUCWTESA-N.
It is a 2'-OMe-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N4-acetyl group masks the cytosine exocyclic amine and is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
5'-O-DMT-N4-acetyl-2'-O-methylcytidine; 5'-O-DMT-2'-OMe-rC(Ac); DMT-2'-OMe-rC(Ac)
Identity and specifications
| Catalog No. | CH64121 |
| CAS No. | 199593-08-3 |
| Molecular Formula | C33H35N3O8 |
| Molecular Weight | 601.6 |
| PubChem CID | 70700566 |
| InChIKey | FINUHOJILDNELQ-PMFUCWTESA-N |
| Purity | >=99% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- Cytosine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Acetyl
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; 1 kg; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
- Stability
- Powder: 3 years at -20 C; 2 years at 4 C; in solvent: 6 months at -80 C; 1 month at -20 C
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 70700566
PubChem · CID 70700566
Frequently Asked Questions
How does it differ from 5'-O-DMT-N4-benzoyl-2'-O-methylcytidine?
Both are 5'-O-DMT-protected 2'-O-methylcytidines with a protected cytosine N4-amine, but this one uses an acetyl group rather than benzoyl. The benzoyl form is cataloged separately.
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