5'-O-DMT-2'-fluoro-2'-deoxyuridine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C30H29FN2O7
- MW
- 548.6
- Purity
- >=99%
5'-O-DMT-2'-fluoro-2'-deoxyuridine is 2'-fluoro-2'-deoxyuridine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group. CH64125 is identified by CAS 146954-74-7, molecular formula C30H29FN2O7, molecular weight 548.6, PubChem CID 7073186, and InChIKey CSSFZSSZXOCCJB-YULOIDQLSA-N.
It is a 2'-F-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance. Because uracil has no exocyclic amine, no base protecting group is required.
Synonyms
5'-O-DMT-2'-fluoro-2'-deoxyuridine; 5'-O-DMT-2'-F-rU; DMT-2'-F-rU
Identity and specifications
| Catalog No. | CH64125 |
| CAS No. | 146954-74-7 |
| Molecular Formula | C30H29FN2O7 |
| Molecular Weight | 548.6 |
| PubChem CID | 7073186 |
| InChIKey | CSSFZSSZXOCCJB-YULOIDQLSA-N |
| Purity | >=99% |
Storage conditions
2-8 C, tightly closed, protected from light and humidity
Technical attributes
- Nucleobase
- Uracil
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 1 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; 1 kg; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white, off-white to faint yellow powder
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- No base protection needed: uracil has no exocyclic amine, so this building block requires no base protecting group.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 7073186
PubChem · CID 7073186
Frequently Asked Questions
How does it differ from 5'-O-DMT-2'-O-methyluridine?
Both are 5'-O-DMT-protected uridines, but this one has a 2'-fluoro modification rather than 2'-O-methyl. The 2'-OMe form is cataloged separately.
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