5'-O-DMT-2'-O-MOE-N4-benzoyl-5-methylcytidine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C41H43N3O9
- MW
- 721.8
- Purity
- >=99%
5'-O-DMT-2'-O-MOE-N4-benzoyl-5-methylcytidine is 2'-O-(2-methoxyethyl)-5-methylcytidine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N4-benzoyl base-protecting group; its base is 5-methylcytosine. CH64127 is identified by CAS 182496-01-1, molecular formula C41H43N3O9, molecular weight 721.8, PubChem CID 15864052, and InChIKey BYSCLUAIKFEFAH-LZURGKRNSA-N.
It is a 2'-O-MOE-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N4-benzoyl group masks the cytosine exocyclic amine and is removed at final deprotection. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
5'-O-DMT-2'-O-MOE-N4-benzoyl-5-methylcytidine; DMT-2'-MOE-5Me-rC(Bz)
Identity and specifications
| Catalog No. | CH64127 |
| CAS No. | 182496-01-1 |
| Molecular Formula | C41H43N3O9 |
| Molecular Weight | 721.8 |
| PubChem CID | 15864052 |
| InChIKey | BYSCLUAIKFEFAH-LZURGKRNSA-N |
| Purity | >=99% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- 5-Methylcytosine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- N4-Benzoyl
- Packaging
- 5 mg; 10 mg; 25 mg; 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; 1 kg; Custom packaging on request
- Physical form
- solid
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies.
- N4-benzoyl base protection (5-methylcytosine base): the benzoyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection; the base is 5-methylcytosine.
Public references
- PubChem Compound 15864052
PubChem · CID 15864052
Frequently Asked Questions
How does it differ from 2'-O-MOE-N4-benzoyl-5-methylcytidine?
Both are N4-benzoyl-protected 2'-MOE 5-methylcytidines, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.
What identifiers should match for CH64127?
Match CAS 182496-01-1, molecular formula C41H43N3O9, molecular weight 721.8, PubChem CID 15864052, and InChIKey BYSCLUAIKFEFAH-LZURGKRNSA-N.
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