5'-O-DMT-2'-O-MOE-N2-isobutyrylguanosine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C38H43N5O9
- MW
- 713.8
- Purity
- >=99%
5'-O-DMT-2'-O-MOE-N2-isobutyrylguanosine is 2'-O-(2-methoxyethyl)guanosine carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group and an N2-isobutyryl base-protecting group. CH64128 is identified by CAS 251647-50-4, molecular formula C38H43N5O9, molecular weight 713.8, PubChem CID 135741311, and InChIKey XDLPRZLZAFJSMA-BEGXHNNXSA-N.
It is a 2'-O-MOE-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle, and the N2-isobutyryl group masks the guanine exocyclic amine and is removed at final deprotection. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
5'-O-DMT-2'-O-MOE-N2-isobutyrylguanosine; DMT-2'-MOE-rG(iBu)
Identity and specifications
| Catalog No. | CH64128 |
| CAS No. | 251647-50-4 |
| Molecular Formula | C38H43N5O9 |
| Molecular Weight | 713.8 |
| PubChem CID | 135741311 |
| InChIKey | XDLPRZLZAFJSMA-BEGXHNNXSA-N |
| Purity | >=99% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- Guanine
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 250 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 250 g; 500 g; 1 kg; Custom packaging on request
- Water content
- <=2.0%
- Physical form
- white to off-white powder
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies.
- N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135741311
PubChem · CID 135741311
Frequently Asked Questions
How does it differ from 2'-O-MOE-N2-isobutyrylguanosine?
Both carry an N2-isobutyryl-protected 2'-O-MOE guanosine, but this one also has a 5'-O-DMT group on the 5'-hydroxyl (as a phosphoramidite precursor). The non-DMT form is cataloged separately.
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