5'-O-DMT-2'-O-MOE-5-methyluridine
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C34H38N2O9
- MW
- 618.7
- Purity
- 99%
5'-O-DMT-2'-O-MOE-5-methyluridine is 2'-O-(2-methoxyethyl)-5-methyluridine (its base is 5-methyluracil) carrying a 5'-O-(4,4'-dimethoxytrityl) (DMT) group. CH64129 is identified by CAS 163759-50-0, molecular formula C34H38N2O9, molecular weight 618.7, PubChem CID 15864049, and InChIKey WIPCVBQXKBWNRC-PBAMLIMUSA-N.
It is a 2'-O-MOE-modified RNA phosphoramidite precursor context: the acid-labile 5'-DMT group is removed at the start of each chain-elongation cycle. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required.
Synonyms
5'-O-DMT-2'-O-MOE-5-methyluridine; DMT-2'-MOE-5Me-rU
Identity and specifications
| Catalog No. | CH64129 |
| CAS No. | 163759-50-0 |
| Molecular Formula | C34H38N2O9 |
| Molecular Weight | 618.7 |
| PubChem CID | 15864049 |
| InChIKey | WIPCVBQXKBWNRC-PBAMLIMUSA-N |
| Purity | 99% |
Storage conditions
2-8 C
Technical attributes
- Nucleobase
- 5-Methyluracil
- 5'-O protection
- DMT (dimethoxytrityl)
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 5 mg; 10 mg; 25 mg; 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 100 g; 1 kg; Custom packaging on request
- Water content
- <=1.0%
- Physical form
- white to off-white powder
Application context
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group protects the 5'-hydroxyl and is removed at the start of each RNA chain-elongation cycle.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies.
- No base protection needed: the 5-methyluracil base has no exocyclic amine, so this building block requires no base protecting group.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 15864049
PubChem · CID 15864049
Frequently Asked Questions
How does it differ from 5'-O-DMT-5-methyl-2'-O-methyluridine?
Both are 5'-O-DMT-protected 5-methyluridines, but this one has a 2'-O-methoxyethyl (2'-MOE) modification, whereas the other has a 2'-O-methyl modification. The 2'-OMe form is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.