2'-Deoxyinosine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C40H47N6O7P
- MW
- 754.8
- Purity
- 99% (NMR)
2'-Deoxyinosine CE Phosphoramidite is the 2'-deoxyinosine building block for DNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyinosine (hypoxanthine base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65008 is identified by CAS 141684-35-7, molecular formula C40H47N6O7P, molecular weight 754.8, PubChem CID 135741319, and InChIKey ZKGZROFWRPJVEB-XZYUMQGLSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, and the acid-labile 5'-DMT is removed at the start of each cycle. Because the hypoxanthine base has no exocyclic amine, no base protecting group is required.
Synonyms
DMT-dI phosphoramidite; 5'-O-DMT-2'-deoxyinosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65008 |
| CAS No. | 141684-35-7 |
| Molecular Formula | C40H47N6O7P |
| Molecular Weight | 754.8 |
| PubChem CID | 135741319 |
| InChIKey | ZKGZROFWRPJVEB-XZYUMQGLSA-N |
| Purity | 99% (NMR) |
Storage conditions
at or below -20 C
Technical attributes
- Monomer class
- DNA CE phosphoramidite
- Nucleobase
- Hypoxanthine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base protection
- Unprotected
- Grade
- Standard
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 100 µmol; Custom packaging on request
- Water content
- K.F. <=0.20% w/w
- Coupling efficiency
- spec >=98.0% and observed 98.0%
- Physical form
- powder
- Stability
- Manufactured 2021-12-30; retest 2025-07-02; expired 2025-12-29
Application context
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
- No base protection needed: the hypoxanthine base has no exocyclic amine, so this monomer requires no base protecting group.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135741319
PubChem · CID 135741319
Frequently Asked Questions
Why does the 2'-deoxyinosine phosphoramidite have no base protecting group?
The hypoxanthine base of 2'-deoxyinosine has no reactive exocyclic amine, so it needs no base protection. The monomer carries only the acid-labile 5'-O-DMT group (removed each cycle) and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-deoxyinosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The 5'-O-DMT-2'-deoxyinosine precursor is cataloged separately.
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