N6-Methyl-2'-deoxyadenosine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C41H50N7O6P
- MW
- 767.9
- Purity
- >=98.0% (HPLC)
N6-Methyl-2'-deoxyadenosine CE Phosphoramidite is a DNA building block for 6mA-modified synthetic DNA research: 5'-O-(4,4'-dimethoxytrityl)-N6-methyl-2'-deoxyadenosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65009 is identified by CAS 105931-58-6, molecular formula C41H50N7O6P, molecular weight 767.9, PubChem CID 86280297, and InChIKey PTWRBAYAPDVZGR-JNTXQERPSA-N.
It is a phosphoramidite coupling monomer for automated solid-phase DNA synthesis in which the base is N6-methyladenine (6mA): the 3'-phosphoramidite is the reactive group that couples to the growing chain, and the acid-labile 5'-DMT is removed at the start of each cycle. The N6-methyl group is the permanent 6mA base modification, not a removable protecting group.
Synonyms
DMT-N6-Me-dA phosphoramidite; 5'-O-DMT-N6-methyl-2'-deoxyadenosine 3'-CE phosphoramidite; 6mA amidite
Identity and specifications
| Catalog No. | CH65009 |
| CAS No. | 105931-58-6 |
| Molecular Formula | C41H50N7O6P |
| Molecular Weight | 767.9 |
| PubChem CID | 86280297 |
| InChIKey | PTWRBAYAPDVZGR-JNTXQERPSA-N |
| Purity | >=98.0% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- Modified DNA CE phosphoramidite
- Nucleobase
- Adenine
- 5'-O protection
- DMT (dimethoxytrityl)
- Base modification
- N6-Methyl (6mA)
- Packaging
- 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; Custom packaging on request
- Physical form
- solid
- Stability
- >=4 years
Application context
- 6mA DNA amidite: provides an N6-methyladenine (6mA) base identity for modified synthetic DNA research.
- DNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 5'-DMT 5'-OH protection: the acid-labile 4,4'-dimethoxytrityl group is removed at the start of each chain-elongation cycle.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 86280297
PubChem · CID 86280297
Frequently Asked Questions
Is the N6-methyl group a protecting group?
No. The N6-methyl is the permanent N6-methyladenine (6mA) base modification. Only the acid-labile 5'-O-DMT group and the 3'-phosphoramidite coupling group are synthetic handles.
What base identity does this amidite provide?
It provides the N6-methyladenine (6mA) base identity for 6mA-modified synthetic DNA research via automated solid-phase phosphoramidite synthesis.
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