2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C47H64N5O9PSi
- MW
- 902.1
- Purity
- 100% (HPLC)
2'-O-TBDMS-Cytidine (Ac) CE Phosphoramidite is the fully protected cytidine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-acetylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65011 is identified by CAS 121058-88-6, molecular formula C47H64N5O9PSi, molecular weight 902.1, PubChem CID 11814945, and InChIKey QKWKXYVKGFKODW-YOEDQOPESA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, the 2'-O-TBDMS protects the ribose 2'-hydroxyl, and the N4-acetyl base group is removed at final deprotection.
Synonyms
DMT-2'-O-TBDMS-rC(Ac) phosphoramidite; 5'-O-DMT-2'-O-TBDMS-N4-acetylcytidine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65011 |
| CAS No. | 121058-88-6 |
| Molecular Formula | C47H64N5O9PSi |
| Molecular Weight | 902.1 |
| PubChem CID | 11814945 |
| InChIKey | QKWKXYVKGFKODW-YOEDQOPESA-N |
| Purity | 100% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- RNA CE phosphoramidite
- Nucleobase
- Cytosine
- 2'-O protection
- TBDMS (tert-butyldimethylsilyl)
- Base protection
- N4-Acetyl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.3% (same-SKU COA specification)
- Physical form
- white to light yellow powder
- Stability
- Manufactured 2022-09-12; retest 2026-03-15; expiry 2026-09-11
Application context
- RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
- 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
- N4-acetyl base protection: the acetyl group masks the cytosine exocyclic amine during synthesis and is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 11814945
PubChem · CID 11814945
Frequently Asked Questions
What are the four functional groups on this RNA amidite?
A 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite (the reactive coupling group), an acid-labile 5'-O-DMT group (removed each cycle), a 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and an N4-acetyl base protection (removed at final deprotection).
How does it differ from the 2'-O-TBDMS-rC(Bz) phosphoramidite?
Both are 2'-O-TBDMS cytidine RNA amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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