2'-O-TBDMS-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C50H68N7O9PSi
- MW
- 970.2
- Purity
- 100.0% (NMR)
2'-O-TBDMS-Guanosine (iBu) CE Phosphoramidite is the fully protected guanosine building block for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N2-isobutyrylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65012 is identified by CAS 147201-04-5, molecular formula C50H68N7O9PSi, molecular weight 970.2, PubChem CID 135587729, and InChIKey PGJKESPHANLWME-FTZVBZPOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase RNA synthesis: the 3'-phosphoramidite is the reactive group that couples to the growing chain, the acid-labile 5'-DMT is removed at the start of each cycle, the 2'-O-TBDMS protects the ribose 2'-hydroxyl, and the N2-isobutyryl base group is removed at final deprotection.
Synonyms
DMT-2'-O-TBDMS-rG(iBu) phosphoramidite; 5'-O-DMT-2'-O-TBDMS-N2-isobutyrylguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65012 |
| CAS No. | 147201-04-5 |
| Molecular Formula | C50H68N7O9PSi |
| Molecular Weight | 970.2 |
| PubChem CID | 135587729 |
| InChIKey | PGJKESPHANLWME-FTZVBZPOSA-N |
| Purity | 100.0% (NMR) |
Storage conditions
-20 C; dry, inert atmosphere
Technical attributes
- Monomer class
- RNA CE phosphoramidite
- Nucleobase
- Guanine
- 2'-O protection
- TBDMS (tert-butyldimethylsilyl)
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 50 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.3%
- Physical form
- white to faint yellow powder
- Stability
- Manufactured 2023-05-30; retest 2026-11-30; expiry 2027-05-29
Application context
- RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase RNA synthesis.
- 5'-DMT + 2'-O-TBDMS protection: the acid-labile 5'-DMT is removed each cycle, while the 2'-O-TBDMS group protects the ribose 2'-hydroxyl during synthesis.
- N2-isobutyryl base protection: the isobutyryl group masks the guanine exocyclic amine during synthesis and is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135587729
PubChem · CID 135587729
Frequently Asked Questions
What are the four functional groups on this RNA amidite?
A 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite (the reactive coupling group), an acid-labile 5'-O-DMT group (removed each cycle), a 2'-O-TBDMS group (protects the ribose 2'-hydroxyl), and an N2-isobutyryl base protection (removed at final deprotection).
How does it relate to 5'-O-DMT-2'-O-TBDMS-N2-isobutyrylguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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