2'-OMe-Cytidine (Ac) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C42H52N5O9P
- MW
- 801.9
- Purity
- >=99% (NMR)
2'-OMe-Cytidine (Ac) CE Phosphoramidite is the 2'-O-methyl cytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-acetyl-2'-O-methylcytidine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65018 is identified by CAS 199593-09-4, molecular formula C42H52N5O9P, molecular weight 801.9, PubChem CID 66764086, and InChIKey WNWUMIPFLOKTEZ-UAQIPLLRSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
DMT-2'-OMe-rC(Ac) phosphoramidite; 5'-O-DMT-N4-acetyl-2'-O-methylcytidine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65018 |
| CAS No. | 199593-09-4 |
| Molecular Formula | C42H52N5O9P |
| Molecular Weight | 801.9 |
| PubChem CID | 66764086 |
| InChIKey | WNWUMIPFLOKTEZ-UAQIPLLRSA-N |
| Purity | >=99% (NMR) |
Storage conditions
-20 C; under inert atmosphere
Technical attributes
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Cytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Acetyl
- Grade
- Standard; TheraPure
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 100 g; Custom packaging on request
- Water content
- <=0.3 wt.% by Karl Fischer
- Physical form
- white to off-white powder
- Stability
- >=4 years
Application context
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited literature discusses 2'-O-methyl residues in relation to nuclease resistance and RNA affinity.
- 5'-DMT + N4-acetyl protection: the acid-labile 5'-DMT is removed each cycle, and the N4-acetyl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 66764086
PubChem · CID 66764086
Frequently Asked Questions
How does it differ from the 2'-OMe-C(Bz) phosphoramidite?
Both are 2'-O-methyl cytidine amidites, but this one uses an N4-acetyl base protection rather than benzoyl. The benzoyl amidite is cataloged separately.
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