2'-OMe-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C45H56N7O9P
- MW
- 869.9
- Purity
- 100% (NMR)
2'-OMe-Guanosine (iBu) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-isobutyryl-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65019 is identified by CAS 150780-67-9, molecular formula C45H56N7O9P, molecular weight 869.9, PubChem CID 135587728, and InChIKey IRRDHRZUOZNWDJ-MLLDKZSOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
DMT-2'-OMe-rG(iBu) phosphoramidite; 5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65019 |
| CAS No. | 150780-67-9 |
| Molecular Formula | C45H56N7O9P |
| Molecular Weight | 869.9 |
| PubChem CID | 135587728 |
| InChIKey | IRRDHRZUOZNWDJ-MLLDKZSOSA-N |
| Purity | 100% (NMR) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Isobutyryl
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- Manufactured 2019-08-16; retest 2023-02-16; expired 2023-08-15
Application context
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135587728
PubChem · CID 135587728
Frequently Asked Questions
How does it relate to 5'-O-DMT-N2-isobutyryl-2'-O-methylguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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