2'-OMe-Guanosine (dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C44H55N8O8P
- MW
- 854.9
- Purity
- >=98.5% (HPLC)
2'-OMe-Guanosine (dmf) CE Phosphoramidite is the 2'-O-methyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N2-dimethylformamidine-2'-O-methylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65021 is identified by CAS 128219-77-2, molecular formula C44H55N8O8P, molecular weight 854.9, PubChem CID 135742510, and InChIKey ZGDBCILCTOHNAB-OWKWDSSOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
DMT-2'-OMe-rG(dmf) phosphoramidite; 5'-O-DMT-N2-dmf-2'-O-methylguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65021 |
| CAS No. | 128219-77-2 |
| Molecular Formula | C44H55N8O8P |
| Molecular Weight | 854.9 |
| PubChem CID | 135742510 |
| InChIKey | ZGDBCILCTOHNAB-OWKWDSSOSA-N |
| Purity | >=98.5% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N2-Dimethylaminomethylene (dmf)
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Application context
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135742510
PubChem · CID 135742510
Frequently Asked Questions
What does this 2'-OMe amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection. The product provides a 2'-O-methylguanosine phosphoramidite identity.
How does it differ from the 2'-OMe-G(iBu) phosphoramidite?
Both are 2'-O-methyl guanosine amidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
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