2'-OMe-Inosine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C41H49N6O8P
- MW
- 784.8
- Purity
- >=98.0% (HPLC)
2'-OMe-Inosine CE Phosphoramidite is the 2'-O-methyl inosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-methylinosine (hypoxanthine base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65022 is identified by CAS 128219-85-2, molecular formula C41H49N6O8P, molecular weight 784.8, PubChem CID 135742511, and InChIKey PVFNTYBGWVZMCX-HLHZJIEZSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the hypoxanthine base has no exocyclic amine, no base protecting group is required. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
DMT-2'-OMe-rI phosphoramidite; 5'-O-DMT-2'-O-methylinosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65022 |
| CAS No. | 128219-85-2 |
| Molecular Formula | C41H49N6O8P |
| Molecular Weight | 784.8 |
| PubChem CID | 135742511 |
| InChIKey | PVFNTYBGWVZMCX-HLHZJIEZSA-N |
| Purity | >=98.0% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- Hypoxanthine
- Sugar modification
- 2'-O-Methyl
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.20% by Karl Fischer
- Physical form
- white/off-white to faint yellow powder; free from visible foreign matter
- Stability
- >=4 years
Application context
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- No base protection needed: the hypoxanthine base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135742511
PubChem · CID 135742511
Frequently Asked Questions
Why does the 2'-OMe-inosine phosphoramidite have no base protecting group?
The hypoxanthine base of inosine has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
How does it differ from the 2'-O-TBDMS-inosine phosphoramidite?
This one has a 2'-O-methyl modification (a permanent methyl on the 2'-oxygen), whereas the 2'-O-TBDMS form protects the 2'-hydroxyl with a silyl group that is later removed. The 2'-O-TBDMS form is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.