2'-OMe-5-Methylcytidine (Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C48H56N5O9P
- MW
- 878.0
- Purity
- >=98.0% (HPLC)
2'-OMe-5-Methylcytidine (Bz) CE Phosphoramidite is the 2'-O-methyl 5-methylcytidine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N4-benzoyl-2'-O-methyl-5-methylcytidine (5-methylcytosine base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65024 is identified by CAS 166593-57-3, molecular formula C48H56N5O9P, molecular weight 878.0, PubChem CID 11967352, and InChIKey GIWYLFDPXKHBKT-AENBYTAOSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N4-benzoyl base group is removed at final deprotection. In the cited literature, 2'-O-methyl ribonucleosides/residues are discussed for nuclease resistance and RNA affinity.
Synonyms
DMT-2'-OMe-5Me-rC(Bz) phosphoramidite; 5'-O-DMT-N4-benzoyl-2'-O-methyl-5-methylcytidine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65024 |
| CAS No. | 166593-57-3 |
| Molecular Formula | C48H56N5O9P |
| Molecular Weight | 878.0 |
| PubChem CID | 11967352 |
| InChIKey | GIWYLFDPXKHBKT-AENBYTAOSA-N |
| Purity | >=98.0% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-methyl RNA CE phosphoramidite
- Nucleobase
- 5-Methylcytosine
- Sugar modification
- 2'-O-Methyl
- Base protection
- N4-Benzoyl
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; Custom packaging on request
- Physical form
- white to off-white powder
- Stability
- powder: 3 years at -20 C; 2 years at 4 C
Application context
- 2'-OMe RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-OMe sugar modification: cited 2'-O-methyl oligonucleotide literature discusses nuclease resistance and RNA affinity.
- 5-Methylcytosine base, N4-benzoyl protected: the base is 5-methylcytosine with an N4-benzoyl protecting group (removed at final deprotection), and the acid-labile 5'-DMT is removed each cycle.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 11967352
PubChem · CID 11967352
Frequently Asked Questions
What base and sugar identity does this amidite provide?
It provides a 2'-O-methyl-5-methylcytidine phosphoramidite identity with a 5-methylcytosine base. The 3'-phosphoramidite is the coupling group, 5'-DMT is removed each cycle, and N4-benzoyl is removed at final deprotection.
How does it differ from the 2'-OMe-C(Bz) phosphoramidite?
Both are N4-benzoyl-protected 2'-O-methyl cytidine amidites, but this one has a 5-methylcytosine base (an extra methyl on the base). The non-methylated form is cataloged separately.
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