2'-F-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C44H53FN7O8P
- MW
- 857.9
- Purity
- 99.9% (HPLC)
2'-F-Guanosine (iBu) CE Phosphoramidite is the 2'-fluoro guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65028 is identified by CAS 144089-97-4, molecular formula C44H53FN7O8P, molecular weight 857.9, PubChem CID 135448972, and InChIKey KJFUMXZZVYMQEU-AOCJBPQJSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. The cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
Synonyms
DMT-2'-F-rG(iBu) phosphoramidite; 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65028 |
| CAS No. | 144089-97-4 |
| Molecular Formula | C44H53FN7O8P |
| Molecular Weight | 857.9 |
| PubChem CID | 135448972 |
| InChIKey | KJFUMXZZVYMQEU-AOCJBPQJSA-N |
| Purity | 99.9% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-fluoro RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-Fluoro-2'-deoxy
- Base protection
- N2-Isobutyryl
- Packaging
- 100 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- <=0.3%
- Physical form
- white to off-white powder
- Stability
- stable under recommended storage conditions
Application context
- 2'-F RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-Fluoro sugar modification: cited oligonucleotide literature discusses 2'-fluoro modifications in relation to RNA affinity and nuclease resistance.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 135448972
PubChem · CID 135448972
Frequently Asked Questions
What does this 2'-F amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-isobutyryl removed at final deprotection. The product provides a 2'-fluoro guanosine phosphoramidite identity.
How does it relate to 5'-O-DMT-2'-fluoro-N2-isobutyryl-2'-deoxyguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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