2'-O-MOE-Guanosine (iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C47H60N7O10P
- MW
- 914.0
- Purity
- >=99% (HPLC)
2'-O-MOE-Guanosine (iBu) CE Phosphoramidite is the 2'-O-methoxyethyl guanosine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-N2-isobutyrylguanosine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65034 is identified by CAS 251647-55-9, molecular formula C47H60N7O10P, molecular weight 914.0, PubChem CID 136745830, and InChIKey LADCDGNEBIQAAU-SBCRAQIVSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
DMT-2'-O-MOE-rG(iBu) phosphoramidite; 5'-O-DMT-2'-O-MOE-N2-isobutyrylguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65034 |
| CAS No. | 251647-55-9 |
| Molecular Formula | C47H60N7O10P |
| Molecular Weight | 914.0 |
| PubChem CID | 136745830 |
| InChIKey | LADCDGNEBIQAAU-SBCRAQIVSA-N |
| Purity | >=99% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-MOE RNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- N2-Isobutyryl
- Grade
- TheraPure
- Packaging
- 50 mg; 200 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.3% by KF
- Physical form
- white to off-white powder
- Stability
- powder: 3 years at -20 C
Application context
- 2'-MOE RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies and as gapmer flank residues.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 136745830
PubChem · CID 136745830
Frequently Asked Questions
What does this 2'-MOE amidite do in RNA synthesis?
Its 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N2-isobutyryl removed at final deprotection. The product provides a 2'-O-methoxyethyl guanosine phosphoramidite identity.
How does it relate to 5'-O-DMT-2'-O-MOE-N2-isobutyrylguanosine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.