2'-O-MOE-5-Methyluridine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C43H55N4O10P
- MW
- 818.9
- Purity
- 100% (HPLC)
2'-O-MOE-5-Methyluridine CE Phosphoramidite is the 2'-O-methoxyethyl 5-methyluridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-5-methyluridine (5-methyluracil base) bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65036 is identified by CAS 163878-63-5, molecular formula C43H55N4O10P, molecular weight 818.9, PubChem CID 92043625, and InChIKey YFRRKZDUDXHJNC-KZQAAKLLSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because the 5-methyluracil base has no exocyclic amine, no base protecting group is required. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
DMT-2'-O-MOE-5Me-rU phosphoramidite; 5'-O-DMT-2'-O-MOE-5-methyluridine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65036 |
| CAS No. | 163878-63-5 |
| Molecular Formula | C43H55N4O10P |
| Molecular Weight | 818.9 |
| PubChem CID | 92043625 |
| InChIKey | YFRRKZDUDXHJNC-KZQAAKLLSA-N |
| Purity | 100% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-MOE RNA CE phosphoramidite
- Nucleobase
- 5-Methyluracil
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 2 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- lot expiration 5 September 2026
Application context
- 2'-MOE RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies and as gapmer flank residues.
- No base protection needed: the 5-methyluracil base has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 92043625
PubChem · CID 92043625
Frequently Asked Questions
Why does this 2'-MOE amidite have no base protecting group?
Its base is 5-methyluracil, which has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-O-MOE-5-methyluridine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.