2'-O-MOE-Uridine CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C42H53N4O10P
- MW
- 804.9
- Purity
- >=98%
2'-O-MOE-Uridine CE Phosphoramidite is the 2'-O-methoxyethyl uridine building block for modified-RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)-uridine bearing a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65038 is identified by CAS 163759-97-5, molecular formula C42H53N4O10P, molecular weight 804.9, PubChem CID 129896826, and InChIKey ZLOKLUONKGURQI-UAQIPLLRSA-N.
Nucleoside phosphoramidites are the coupling monomers used in automated solid-phase oligonucleotide synthesis: the 3'-phosphoramidite couples to the growing chain and the acid-labile 5'-DMT is removed each cycle. Because uracil has no exocyclic amine, no base protecting group is required. In the cited structure and stability literature, 2'-O-MOE ribonucleosides/residues are discussed in RNA-affinity and nuclease-resistance studies and as flanking residues in gapmer antisense oligonucleotides.
Synonyms
DMT-2'-O-MOE-rU phosphoramidite; 5'-O-DMT-2'-O-MOE-uridine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65038 |
| CAS No. | 163759-97-5 |
| Molecular Formula | C42H53N4O10P |
| Molecular Weight | 804.9 |
| PubChem CID | 129896826 |
| InChIKey | ZLOKLUONKGURQI-UAQIPLLRSA-N |
| Purity | >=98% |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 2'-O-MOE RNA CE phosphoramidite
- Nucleobase
- Uracil
- Sugar modification
- 2'-O-(2-Methoxyethyl) (MOE)
- Base protection
- Unprotected
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
- Stability
- >=4 years
Application context
- 2'-MOE RNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- 2'-MOE sugar modification: cited structure and stability literature discusses 2'-O-MOE residues in RNA-affinity and nuclease-resistance studies and as gapmer flank residues.
- No base protection needed: uracil has no exocyclic amine, so this monomer requires no base protecting group (only the acid-labile 5'-DMT, removed each cycle).
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 129896826
PubChem · CID 129896826
Frequently Asked Questions
Why does the 2'-MOE-uridine phosphoramidite have no base protecting group?
Uracil has no reactive exocyclic amine, so it needs no base protection. The monomer carries the acid-labile 5'-O-DMT group, removed each cycle, and the 3'-phosphoramidite coupling group.
How does it relate to 5'-O-DMT-2'-O-MOE-uridine?
It is the phosphoramidite of that nucleoside: the 3'-hydroxyl has been converted to a 2-cyanoethyl N,N-diisopropyl phosphoramidite. The precursor is cataloged separately.
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