LNA-A(Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C48H52N7O8P
- MW
- 885.9
- Purity
- >=99% (HPLC)
LNA-A(Bz) CE Phosphoramidite is the locked nucleic acid (LNA) adenosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N6-benzoyladenosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65045 is identified by CAS 206055-79-0, molecular formula C48H52N7O8P, molecular weight 885.9, PubChem CID 13009021, and InChIKey HPIDKMAOZZZGOP-LIUGLABVSA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection.
Synonyms
DMT-LNA-A(Bz) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N6-benzoyladenosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65045 |
| CAS No. | 206055-79-0 |
| Molecular Formula | C48H52N7O8P |
| Molecular Weight | 885.9 |
| PubChem CID | 13009021 |
| InChIKey | HPIDKMAOZZZGOP-LIUGLABVSA-N |
| Purity | >=99% (HPLC) |
Storage conditions
-20 C; protect from moisture and sunlight
Technical attributes
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- Adenine
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- N6-benzoyl
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- NMT 0.50%
- Physical form
- white to off-white solid; free from foreign particles
- Stability
- >=4 years
Application context
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- 5'-DMT + N6-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 13009021
PubChem · CID 13009021
Frequently Asked Questions
What is LNA and what does this amidite do?
LNA (locked nucleic acid) contains a rigid 2'-O,4'-C-methylene bridge. This amidite's 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N6-benzoyl removed at final deprotection.
How does LNA differ from 2'-OMe or 2'-F modifications?
They are different sugar-modification chemistries: LNA locks the sugar with a 2'-O,4'-C-methylene bridge, while 2'-OMe and 2'-F are 2'-substituted ribose/deoxyribose analogs. The 2'-OMe and 2'-F amidites are cataloged separately.
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