LNA-G(iBu) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C45H54N7O9P
- MW
- 867.9
- Purity
- >98.5% (HPLC)
LNA-G(iBu) CE Phosphoramidite is the locked nucleic acid (LNA) guanosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N2-isobutyrylguanosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65047 is identified by CAS 206055-77-8, molecular formula C45H54N7O9P, molecular weight 867.9, PubChem CID 136760121, and InChIKey UUBFSNDSLDOIDQ-NIQOLRRHSA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Synonyms
DMT-LNA-G(iBu) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N2-isobutyrylguanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65047 |
| CAS No. | 206055-77-8 |
| Molecular Formula | C45H54N7O9P |
| Molecular Weight | 867.9 |
| PubChem CID | 136760121 |
| InChIKey | UUBFSNDSLDOIDQ-NIQOLRRHSA-N |
| Purity | >98.5% (HPLC) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- N2-isobutyryl
- Grade
- N (Normal)
- Packaging
- 1 g; 10 g; 100 g; 500 g; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- white to off-white powder
Application context
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- 5'-DMT + N2-isobutyryl protection: the acid-labile 5'-DMT is removed each cycle, and the N2-isobutyryl base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 136760121
PubChem · CID 136760121
Frequently Asked Questions
What residue identity does this LNA amidite provide?
It provides the locked (LNA) guanosine residue identity through its 3'-phosphoramidite coupling group, with 5'-DMT removed each cycle and N2-isobutyryl removed at final deprotection.
How does it differ from the LNA-A(Bz) amidite?
Both are LNA (locked) phosphoramidites, but this one has an N2-isobutyryl-protected guanine, whereas the LNA-A(Bz) amidite has an N6-benzoyl adenine. Each LNA base is cataloged separately.
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