LNA-G(dmf) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C44H53N8O8P
- MW
- 852.9
- Purity
- 100% (HPLC)
LNA-G(dmf) CE Phosphoramidite is the locked nucleic acid (LNA) guanosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N2-dimethylformamidine-guanosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65049 is identified by CAS 709641-79-2, molecular formula C44H53N8O8P, molecular weight 852.9, PubChem CID 161667440, and InChIKey ACJXYPZQPQPGNE-KPKUBJDASA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N2-dmf base group is removed at final deprotection.
Synonyms
DMT-LNA-G(dmf) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N2-dmf-guanosine 3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65049 |
| CAS No. | 709641-79-2 |
| Molecular Formula | C44H53N8O8P |
| Molecular Weight | 852.9 |
| PubChem CID | 161667440 |
| InChIKey | ACJXYPZQPQPGNE-KPKUBJDASA-N |
| Purity | 100% (HPLC) |
Storage conditions
-20 C; dry and inert atmosphere
Technical attributes
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- Guanine
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- N2-dimethylformamidine
- Grade
- N (Normal)
- Packaging
- 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; 500 g; Custom packaging on request
- Water content
- K.F. <=0.50% w/w
- Physical form
- white, off-white to faint yellow powder, free from visible foreign matter
- Stability
- >=4 years
Application context
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- 5'-DMT + N2-dmf protection: the acid-labile 5'-DMT is removed each cycle, and the N2-dimethylformamidine base group is removed at final deprotection.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 161667440
PubChem · CID 161667440
Frequently Asked Questions
What residue identity does this LNA amidite provide?
It provides the locked (LNA) guanosine residue identity through its 3'-phosphoramidite coupling group, with 5'-DMT removed each cycle and N2-dmf removed at final deprotection.
How does it differ from the LNA-G(iBu) amidite?
Both are LNA (locked) guanosine phosphoramidites, but this one uses an N2-dimethylformamidine (dmf) base protection rather than isobutyryl. The isobutyryl amidite is cataloged separately.
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