dTTP, 100 mM Solution
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H14N2Na3O14P3
- MW
- 548.11
- Purity
- 99.98%
dTTP, 100 mM Solution is thymidine-5'-triphosphate (dTTP, also written TTP) listed as a 100 mM aqueous solution of the trisodium salt. Key identifiers include CAS 27821-54-1, molecular formula C10H14N2Na3O14P3, and molecular weight 548.11 (PubChem CID 5311491).
dTTP is one of the four canonical deoxynucleoside triphosphates (dNTPs). DNA polymerases catalyze the template-directed polymerization of dNTP substrates onto the 3'-end of a growing DNA primer strand, so dTTP supplies the thymine (T) residue during enzymatic DNA synthesis — for example in PCR, primer extension, DNA sequencing, and nick translation.
Synonyms
thymidine 5'-triphosphate trisodium salt; TTP; dTTP·3Na, 100 mM aqueous solution
Identity and specifications
| Catalog No. | CH66004 |
| CAS No. | 27821-54-1 |
| Molecular Formula | C10H14N2Na3O14P3 (trisodium salt) |
| Molecular Weight | 548.11 |
| PubChem CID | 5311491 |
| InChIKey | KNVJTOALWGZWAI-SPSULGLQSA-K |
| Concentration | 100 mM aqueous solution |
| Purity | 99.98% |
Storage conditions
-20 C
Technical attributes
- Nucleotide class
- dNTP
- Nucleobase
- Thymine
- 5'-phosphate state
- Triphosphate
- Grade
- PCR Grade
- Packaging
- 100 µL; 200 µL; 500 µL; 1 mL; 5 mL; 10 mL; 50 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- colorless transparent solution
- Stability
- 2 years at -20 C as a solution
Application context
- Thymine dNTP substrate: supplies the thymine (T) residue during enzymatic, template-directed DNA synthesis by DNA polymerases.
- Common reactions: PCR, primer extension, DNA sequencing, nick translation, and other polymerase-based DNA synthesis reactions.
- Solution presentation: listed as a 100 mM aqueous solution of the trisodium salt.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 5311491
PubChem · CID 5311491
Frequently Asked Questions
What is dTTP used for?
dTTP (thymidine triphosphate) is the thymine deoxynucleoside triphosphate. DNA polymerases use it as a substrate to add thymine (T) residues during enzymatic, template-directed DNA synthesis — for example in PCR, primer extension, sequencing, and nick translation.
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