5-aa-2'-dUTP (Aminoallyl-dUTP)
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Purity
- >=98%
5-aa-2'-dUTP is 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate, commonly described as aminoallyl-dUTP. It is a modified deoxynucleotide triphosphate that carries a primary amine on a 5-aminoallyl linker at the C5 position of uracil. Key identifiers for this catalog entry are CHEMOS CH66011 and CAS 936327-10-5.
In enzymatic DNA labeling workflows, aminoallyl-dUTP can be incorporated by DNA polymerases during template-directed DNA synthesis. The introduced primary amine can then react with amine-reactive reporters such as NHS-ester dyes, allowing post-synthetic labeling of DNA products. This page keeps the identity boundary to the supplier catalog name, CAS number, and literature-supported aminoallyl-dUTP use context.
Synonyms
aminoallyl-dUTP; 5-(3-aminoallyl)-2'-deoxyuridine-5'-triphosphate; AA-dUTP
Identity and specifications
| Catalog No. | CH66011 |
| Product Name | 5-aa-2'-dUTP (Aminoallyl-dUTP) |
| CAS No. | 936327-10-5 |
| Product Type | Amine-modified deoxynucleotide triphosphate |
| Labeling Handle | 5-aminoallyl primary amine |
| Purity | >=98% |
Storage conditions
freeze below -15 C; minimize light exposure
Technical attributes
- Nucleotide class
- dNTP
- Parent nucleotide
- dUTP
- Base modification
- 5-(3-Aminoallyl)
- Reactive handle
- Primary amine
- Packaging
- 1 mg; 5 mg; 25 mg; 50 mg; 100 mg; 5 x 1 mg; Custom packaging on request
- Solution concentration
- 4 mM
- Physical form
- colorless liquid
- Stability
- 6 months upon receiving
Application context
- Amine handle in DNA: used as an aminoallyl-modified dUTP to introduce a primary amine into enzymatically synthesized DNA.
- Post-synthetic labeling: the aminoallyl handle can react with amine-reactive reporters such as NHS-ester dyes.
- Probe preparation: relevant to DNA labeling workflows such as PCR, reverse-transcription labeling, and microarray probe preparation.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Aminoallyl-dUTP sodium salt, catalog A0410
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
Frequently Asked Questions
What is 5-aa-2'-dUTP used for?
5-aa-2'-dUTP is an aminoallyl-modified dUTP used to introduce a primary amine into enzymatically synthesized DNA for post-synthetic labeling.
Why use an aminoallyl handle?
The aminoallyl group provides a small primary amine handle that can react with amine-reactive reporters such as NHS-ester dyes after nucleotide incorporation.
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