3'-O-allyl-2'-dATP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Purity
- >=98%
3'-O-allyl-2'-dATP is a 3'-O-allyl modified dATP reversible terminator. The 3'-hydroxyl is capped with a reversible blocking group so that polymerase extension stops after a single incorporation cycle. Key identifiers are CHEMOS CH66022, CAS 917766-44-0, molecular formula C13H20N5O12P3, and molecular weight 531.25.
3'-O-modified nucleotides are used in cyclic reversible-terminator sequencing-by-synthesis workflows: DNA polymerase incorporates the nucleotide, extension pauses after one base, and cap removal regenerates the 3'-OH so synthesis can continue.
Synonyms
3'-O-allyl-dATP; 3'-O-(prop-2-en-1-yl)-2'-deoxyadenosine-5'-triphosphate
Identity and specifications
| Catalog No. | CH66022 |
| Product Name | 3'-O-allyl-2'-dATP |
| CAS No. | 917766-44-0 |
| Molecular Formula | C13H20N5O12P3 |
| Molecular Weight | 531.25 |
| Product Type | 3'-O-allyl reversible-terminator dATP |
| Base Added | Adenine |
| Purity | >=98% |
Technical attributes
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dATP
- 3'-O blocking group
- Allyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- Custom packaging on request
Application context
- Reversible terminator nucleotide: used as the adenine member of a 3'-O-blocked dNTP set.
- Sequencing-by-synthesis context: incorporation pauses extension for one cycle before cap removal allows synthesis to continue.
- Modified nucleotide cataloging: relevant to oligonucleotide synthesis, sequencing reagent, and modified dNTP product organization.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- 3'-O-Allyl-dATP, catalog HY-185480
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
Frequently Asked Questions
What is 3'-O-allyl-2'-dATP?
3'-O-allyl-2'-dATP is a 3'-O-allyl modified dATP used as a reversible-terminator nucleotide in sequencing-by-synthesis research workflows.
How does a reversible terminator work?
DNA polymerase incorporates the nucleotide, the 3'-O blocking group pauses further extension, and removal of the cap regenerates the 3'-OH so synthesis can continue.
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