3'-O-allyl-2'-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C13H21N2O14P3
- MW
- 522.23
- Purity
- >=98%
3'-O-allyl-2'-dTTP is 3'-O-allyl-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with an allyl (prop-2-en-1-yl) group. Key identifiers include CAS 917766-47-3, molecular formula C13H21N2O14P3, and molecular weight 522.23 (PubChem CID 77134385).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-allyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyms
3'-O-allyl-dTTP; 3'-O-(prop-2-en-1-yl)-thymidine-5'-triphosphate
Identity and specifications
| Catalog No. | CH66025 |
| CAS No. | 917766-47-3 |
| Molecular Formula | C13H21N2O14P3 |
| Molecular Weight | 522.23 |
| PubChem CID | 77134385 |
| InChIKey | JGJOVFWVTMUSDJ-HBNTYKKESA-N |
| Purity | >=98% |
Technical attributes
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Allyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- Custom packaging on request
Application context
- Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-allyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Thymine building block: supplies the thymine reversible terminator in a four-nucleotide SBS set.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 77134385
PubChem · CID 77134385
Frequently Asked Questions
What is a 3'-O-allyl reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with an allyl group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the allyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the thymine (T) reversible terminator — 3'-O-allyl-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-allyl terminators it forms a four-nucleotide set for sequencing-by-synthesis.
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