3'-O-NH2-2'-dCTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C9H17N4O13P3
- MW
- 482.17
- Purity
- 99.0%
3'-O-NH2-2'-dCTP is 3'-O-amino-2'-deoxycytidine-5'-triphosphate: a reversible-terminator dCTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. Key identifiers include CAS 1220515-75-2, molecular formula C9H17N4O13P3, and molecular weight 482.17 (PubChem CID 87229304).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-amino cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyms
3'-O-amino-dCTP; 3'-O-(aminooxy)-2'-deoxycytidine-5'-triphosphate
Identity and specifications
| Catalog No. | CH66027 |
| CAS No. | 1220515-75-2 |
| Molecular Formula | C9H17N4O13P3 |
| Molecular Weight | 482.17 |
| PubChem CID | 87229304 |
| InChIKey | VECVOKKASOJPEY-SHYZEUOFSA-N |
| Purity | 99.0% |
Storage conditions
material in solvent at -80 C
Technical attributes
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dCTP
- 3'-O blocking group
- Amino
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 1 mL; 5 mL; 10 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- liquid; colorless to light yellow
- Stability
- 1 year in solvent at -80 C
Application context
- Reversible terminator: DNA polymerase incorporates it, adding one cytosine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-amino cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Cytosine building block: supplies the cytosine reversible terminator in a four-nucleotide SBS set.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 87229304
PubChem · CID 87229304
Frequently Asked Questions
What is a 3'-O-amino reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the 3'-O-amino cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the cytosine (C) reversible terminator — 3'-O-amino-2'-deoxycytidine-5'-triphosphate.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.