3'-O-NH2-2'-dTTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C10H18N3O14P3
- MW
- 497.18
- Purity
- 99.00%
3'-O-NH2-2'-dTTP is 3'-O-amino-thymidine-5'-triphosphate: a reversible-terminator dTTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. Key identifiers include CAS 1159990-37-0, molecular formula C10H18N3O14P3, and molecular weight 497.18 (PubChem CID 86760875).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-amino cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyms
3'-O-amino-dTTP; 3'-O-(aminooxy)-thymidine-5'-triphosphate
Identity and specifications
| Catalog No. | CH66029 |
| CAS No. | 1159990-37-0 |
| Molecular Formula | C10H18N3O14P3 |
| Molecular Weight | 497.18 |
| PubChem CID | 86760875 |
| InChIKey | WTXJWVBIEUJGOO-XLPZGREQSA-N |
| Purity | 99.00% |
Storage conditions
material in solvent at -80 C
Technical attributes
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dTTP
- 3'-O blocking group
- Amino
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 100 µL; 500 µL; 1 mL; 5 mL; 10 mL; Custom packaging on request
- Solution concentration
- 100 mM
- Physical form
- liquid; colorless to light yellow
- Stability
- 1 year in solvent at -80 C
Application context
- Reversible terminator: DNA polymerase incorporates it, adding one thymine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-amino cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Thymine building block: supplies the thymine reversible terminator, completing the four-nucleotide (A/C/G/T) 3'-O-amino SBS set.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 86760875
PubChem · CID 86760875
Frequently Asked Questions
What is a 3'-O-amino reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small 3'-O-amino group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the 3'-O-amino cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the thymine (T) reversible terminator — 3'-O-amino-thymidine-5'-triphosphate. Together with the A, C, and G 3'-O-amino terminators it forms a four-nucleotide set for sequencing-by-synthesis.
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