3'-O-Ac-2'-dCTP
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C11H18N3O14P3
- MW
- 509.19
- Purity
- >95% (HPLC)
3'-O-Ac-2'-dCTP is 3'-O-acetyl-2'-deoxycytidine-5'-triphosphate: a reversible-terminator dCTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. Key identifiers include CAS 90290-80-5, molecular formula C11H18N3O14P3, and molecular weight 509.19 (PubChem CID 172873581).
3'-O-modified nucleotides — the 3'-OH capped with a small reversible moiety — are still recognized by DNA polymerase as substrates and act as reversible terminators: incorporation adds one base and stops extension. After each incorporation and readout, the 3'-O-acetyl cap is removed to regenerate the 3'-OH so the polymerase reinitiates — the basis of cyclic reversible-terminator sequencing-by-synthesis.
Synonyms
3'-O-acetyl-dCTP; 3'-O-(acetyl)-2'-deoxycytidine-5'-triphosphate
Identity and specifications
| Catalog No. | CH66031 |
| CAS No. | 90290-80-5 |
| Molecular Formula | C11H18N3O14P3 |
| Molecular Weight | 509.19 |
| PubChem CID | 172873581 |
| InChIKey | LKLQSAZKIAYJCI-QXFUBDJGSA-N |
| Purity | >95% (HPLC) |
Storage conditions
-70 C
Technical attributes
- Nucleotide class
- Reversible-terminator dNTP
- Parent nucleotide
- dCTP
- 3'-O blocking group
- Acetyl
- Deblocking outcome
- Regenerates the 3'-OH
- Packaging
- 10 mg; 50 mg; 5 umol (~2.5 mg) in 500 uL vial; Custom packaging on request
- Solution concentration
- 10 mM
- Physical form
- 10 mM aqueous sodium-salt solution
- Stability
- slowly decomposes at ambient temperature; thaw only before use and avoid repeated freeze/thaw cycles
Application context
- Reversible terminator: DNA polymerase incorporates it, adding one cytosine and stopping extension for that cycle.
- Cap removal reinitiates: removing the 3'-O-acetyl cap regenerates the 3'-OH so synthesis continues — enabling cyclic sequencing-by-synthesis.
- Cytosine building block: supplies the cytosine reversible terminator in a four-nucleotide SBS set.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 172873581
PubChem · CID 172873581
Frequently Asked Questions
What is a 3'-O-acetyl reversible terminator?
It is a dNTP whose 3'-hydroxyl is capped with a small acetyl (ester) group. DNA polymerase still incorporates it, but the cap stops further extension after one base. Removing the acetyl cap regenerates the 3'-OH so synthesis can continue — the principle of cyclic reversible-terminator sequencing-by-synthesis.
Which base does this terminator add?
It is the cytosine (C) reversible terminator — 3'-O-acetyl-2'-deoxycytidine-5'-triphosphate.
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