DMT-2'-O-TBDMS-N1-Me-Pseudouridine-CE
3-[[(2R,3R,4S,5S)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[tert-butyl(dimethyl)silyl]oxy-5-(1-methyl-2,4-dioxopyrimidin-5-yl)oxolan-3-yl]oxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- Chemical Name
- 3-[[(2R,3R,4S,5S)-2-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[tert-butyl(dimethyl)silyl]oxy-5-(1-methyl-2,4-dioxopyrimidin-5-yl)oxolan-3-yl]oxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
- MF
- C46H63N4O9PSi
- MW
- 875.1
- Purity
- >=98.0% (NMR)
DMT-2'-O-TBDMS-N1-Me-Pseudouridine-CE is mapped to a protected N1-methylpseudouridine CE phosphoramidite. PubChem CID 167312621 records CAS 875302-45-7, molecular formula C46H63N4O9PSi, molecular weight 875.1, exact mass 874.41019313, and InChIKey ZDZLHJPETUXZJT-NSASEYFNSA-N.
The molecule combines a 5'-DMT-protected hydroxyl, a 2'-O-TBDMS-protected ribose hydroxyl, and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. This makes it a protected N1-methylpseudouridine monomer for research workflows involving modified RNA oligonucleotide synthesis.
Synonyms
5'-DMT-2'-O-TBDMS-N1-Methyl-PseudoUridine-CE-Phosphoramidite; N1-Methylpseudouridine CEP; DMT-2'-O-TBDMS-N1-Me-Pseudouridine-CE (source name)
Identity and specifications
| Catalog No. | CH70379 |
| Product Name | DMT-2'-O-TBDMS-N1-Me-Pseudouridine-CE |
| CAS No. | 875302-45-7 |
| PubChem CID | 167312621 |
| Molecular Formula | C46H63N4O9PSi |
| Molecular Weight | 875.1 |
| Exact Mass | 874.41019313 |
| InChIKey | ZDZLHJPETUXZJT-NSASEYFNSA-N |
| Synonyms | 5'-DMT-2'-O-TBDMS-N1-Methyl-PseudoUridine-CE-Phosphoramidite; N1-Methylpseudouridine CEP |
| Purity | >=98.0% (NMR) |
Storage conditions
-20 C
Technical attributes
- Monomer class
- N1-methylpseudouridine CE phosphoramidite
- Nucleoside identity
- N1-Methylpseudouridine
- 2'-O protection
- TBDMS
- Coupling group
- 3'-O-CE phosphoramidite
- Packaging
- 25 mg; 250 mg; 250 mg (ABI 10 mL / 20 mm septum); 250 umol (ABI 10 mL / 20 mm septum); 500 mg (ABI 10 mL / 20 mm septum); Custom packaging on request
- Physical form
- White - pale yellow, crystals - powder
- Stability
- Powder: 2 years; in solution at -20 C: 6 months; in solution at -80 C: 1 year
Application context
- Protected CE phosphoramidite monomer: a protected N1-methylpseudouridine building block for solid-phase RNA oligonucleotide synthesis research.
- 2'-O-TBDMS RNA building block: the TBDMS group protects the ribose 2'-hydroxyl in RNA phosphoramidite workflows.
- Modified pseudouridine oligonucleotide research: supports research contexts requiring an N1-methylpseudouridine phosphoramidite identity.
Additional material information
Uses
Use as a research phosphoramidite identity in workflows involving protected N1-methylpseudouridine monomers, modified RNA oligonucleotide synthesis, or 2'-O-TBDMS RNA building-block selection.
Characteristics
CH70379 is a phosphoramidite form of protected N1-methylpseudouridine. It is distinct from protected nucleoside intermediates that do not carry the CE phosphoramidite group.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 167312621
PubChem · CID 167312621
Frequently Asked Questions
What is DMT-2'-O-TBDMS-N1-Me-Pseudouridine-CE?
It is a protected N1-methylpseudouridine CE phosphoramidite. PubChem CID 167312621 maps CAS 875302-45-7 to this protected phosphoramidite identity.
What protecting groups does this monomer contain?
The PubChem structure contains a 5'-DMT-protected hydroxyl and a 2'-O-TBDMS-protected ribose hydroxyl, plus a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group.
Is this the same as 5'-O-DMT-2'-O-TBDMS-N1-methyl-pseudouridine?
No automatic equivalence is assumed. CH70379 is the CE phosphoramidite form under CAS 875302-45-7; the protected nucleoside without the phosphoramidite group is a different identity.
Similar Products
Need Help with Material Selection?
CHEMOS can review product fit, target structure, route questions, analytical expectations, and project-specific supply needs.