Spacer-9 (Triethylene Glycol) Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C36H49N2O7P
- MW
- 652.8
- Purity
- >98%
Spacer-9 (Triethylene Glycol) Phosphoramidite is a non-nucleotide (abasic) spacer building block: a triethylene glycol chain carrying a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 146668-73-7, molecular formula C36H49N2O7P, molecular weight 652.8, and PubChem CID 56846264.
Coupling inserts a flexible, polar non-nucleotide ethylene-glycol spacer into the oligonucleotide backbone in place of a nucleotide — ethylene glycol chains can be incorporated into oligonucleotides this way (for example, joining two complementary segments into a hairpin loop, or introducing a defined gap). The spacer couples through its 3'-phosphoramidite, and its 5'-DMT is removed each cycle so chain assembly continues.
Synonyms
Spacer 9; triethylene glycol spacer phosphoramidite; DMT-O-(triethylene glycol)-3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65099 |
| CAS No. | 146668-73-7 |
| Molecular Formula | C36H49N2O7P |
| Molecular Weight | 652.8 |
| PubChem CID | 56846264 |
| InChIKey | PEJGGZVVBQPTRG-UHFFFAOYSA-N |
| Purity | >98% |
Storage conditions
-20 C
Technical attributes
- Reagent class
- Non-nucleotide spacer phosphoramidite
- Spacer type
- Triethylene glycol
- Ethylene glycol units
- 3
- End protection
- 5'-O-DMT
- Grade
- N (Normal)
- Packaging
- 250 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 1 kg; 5 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colorless oily liquid
Application context
- Flexible ethylene-glycol spacer: introduces a polar, flexible triethylene glycol abasic spacer into the oligonucleotide backbone in place of a nucleotide.
- Segment linker / loop: ethylene glycol chains can join two complementary segments into a hairpin loop or introduce a defined gap.
- Chain-compatible: couples through a 3'-phosphoramidite and carries a 5'-DMT that is removed each cycle, so synthesis can continue past the spacer.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 56846264
PubChem · CID 56846264
Frequently Asked Questions
What is the Spacer-9 (triethylene glycol) phosphoramidite?
It is a non-nucleotide (abasic) spacer built on a triethylene glycol chain. Coupling inserts a flexible, polar ethylene-glycol spacer into the oligonucleotide backbone in place of a nucleotide, for joining segments or introducing a defined gap.
How does it differ from the alkyl (C-series) spacers?
It uses an ethylene glycol (ether) chain rather than a pure hydrocarbon chain, making it more polar and flexible; the extra oxygens are reflected in its formula (C36H49N2O7P). Otherwise it couples the same way, via a 3'-phosphoramidite with a 5'-DMT removed each cycle.
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