Spacer-18 (Hexaethylene Glycol) Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C42H61N2O10P
- MW
- 784.9
- Purity
- 99%
Spacer-18 (Hexaethylene Glycol) Phosphoramidite is a non-nucleotide (abasic) spacer building block: a hexaethylene glycol chain carrying a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 125607-09-2, molecular formula C42H61N2O10P, molecular weight 784.9, and PubChem CID 22904875.
Coupling inserts a long, flexible, polar non-nucleotide ethylene-glycol spacer into the oligonucleotide backbone in place of nucleotides. The cited hexaethylene glycol literature describes this chain type joining two complementary segments into a hairpin. The spacer couples through its 3'-phosphoramidite, and its 5'-DMT is removed each cycle so chain assembly continues.
Synonyms
Spacer 18; hexaethylene glycol spacer phosphoramidite; DMT-O-(hexaethylene glycol)-3'-CE phosphoramidite
Identity and specifications
| Catalog No. | CH65100 |
| CAS No. | 125607-09-2 |
| Molecular Formula | C42H61N2O10P |
| Molecular Weight | 784.9 |
| PubChem CID | 22904875 |
| InChIKey | ZTCKUVQHKIMCLI-UHFFFAOYSA-N |
| Purity | 99% |
Storage conditions
-20 C in dark; desiccate; protect from prolonged light
Technical attributes
- Reagent class
- Non-nucleotide spacer phosphoramidite
- Spacer type
- Hexaethylene glycol
- Ethylene glycol units
- 6
- End protection
- 5'-O-DMT
- Grade
- N (Normal)
- Packaging
- 10 mg; 25 mg; 50 mg; 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 10 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colorless/yellowish oil
- Stability
- 12 months after receipt under stated storage
Application context
- Long flexible ethylene-glycol spacer: introduces a long, polar hexaethylene glycol abasic spacer into the oligonucleotide backbone in place of nucleotides.
- Hairpin loop: a hexaethylene glycol chain can join two complementary segments into a hairpin loop.
- Chain-compatible: couples through a 3'-phosphoramidite and carries a 5'-DMT that is removed each cycle, so synthesis can continue past the spacer.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 22904875
PubChem · CID 22904875
Frequently Asked Questions
What is the Spacer-18 (hexaethylene glycol) phosphoramidite?
It is a non-nucleotide (abasic) spacer built on a hexaethylene glycol chain. Coupling inserts a long, flexible, polar ethylene-glycol spacer into the oligonucleotide backbone in place of nucleotides.
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