Chemical Phosphorylation Reagent II
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C39H51N2O9P
- MW
- 722.8
- Purity
- 98% (HPLC)
Chemical Phosphorylation Reagent II is a non-nucleoside building block for introducing a phosphate group on an oligonucleotide during synthesis: a phosphoramidite carrying a protected phosphate (with a 5'-O-(4,4'-dimethoxytrityl) handle) plus a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 171285-25-9, molecular formula C39H51N2O9P, molecular weight 722.8, and PubChem CID 10283713.
Like the first Chemical Phosphorylation Reagent, it couples at the oligonucleotide terminus like any amidite; after the phosphate protecting group is removed during deprotection, it leaves a phosphate monoester — for example a 5'-phosphate — chemically, without an enzymatic step. The "II" name distinguishes this no-sulfur protected-phosphate structure from CH65101.
Synonyms
CPR II; Chemical Phosphorylation Reagent II; 5'-phosphate-ON reagent
Identity and specifications
| Catalog No. | CH65102 |
| CAS No. | 171285-25-9 |
| Molecular Formula | C39H51N2O9P |
| Molecular Weight | 722.8 |
| PubChem CID | 10283713 |
| InChIKey | IEKIULVSVQLVMS-UHFFFAOYSA-N |
| Purity | 98% (HPLC) |
Storage conditions
-20 C; dark; desiccated
Technical attributes
- Reagent class
- Non-nucleoside phosphorylation phosphoramidite
- Installation site
- Oligonucleotide terminus
- Installed group
- Phosphate monoester after deprotection
- Structure distinction
- No-sulfur protected-phosphate structure
- Packaging
- 10 mg; 100 mg; 250 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 10 kg; 50 kg; 100 µmol; Custom packaging on request
- Water content
- <=0.5%
- Physical form
- colorless semisolid
- Stability
- Product: 12 months after receipt; diluted reagent: <=24 hours under anhydrous conditions; transport: up to 3 weeks at room temperature
Application context
- Chemical Phosphorylation Reagent II reference: use this chemical phosphorylation reagent record for oligonucleotide phosphorylation reagent catalog work.
- Identifier cross-check: compare CAS 171285-25-9, PubChem CID 10283713, formula C39H51N2O9P, MW 722.8, and the listed InChIKey for catalog identity matching.
- Inquiry preparation: include catalog number CH65102, the product name, and the identity-matching identifiers when preparing a CHEMOS product inquiry.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 10283713
PubChem · CID 10283713
Frequently Asked Questions
What does Chemical Phosphorylation Reagent II do?
It provides a phosphate group on an oligonucleotide chemically, during solid-phase synthesis, instead of using an enzyme (kinase). It couples like an amidite and, after deprotection, leaves a phosphate monoester — for example a 5'-phosphate.
How does it differ from the first Chemical Phosphorylation Reagent?
Both provide a terminal phosphate through the same protected-phosphate phosphoramidite concept. The 'II' name distinguishes a no-sulfur structure (C39H51N2O9P) from the sulfonyl-containing first reagent.
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