2'-O-TBDMS-rA (Bz) 3'-Succinate, Triethylammonium Salt
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C48H53N5O10Si
- MW
- 888.0
- Purity
- 98%
2'-O-TBDMS-rA (Bz) 3'-Succinate, Triethylammonium Salt is a support-loading precursor for RNA synthesis: 5'-O-(4,4'-dimethoxytrityl)-N6-benzoyl-2'-O-(tert-butyldimethylsilyl)-adenosine 3'-O-succinate, listed as its triethylammonium salt. Its PubChem-backed succinate identity includes CAS 131316-88-6, molecular formula C48H53N5O10Si, molecular weight 888.0, and PubChem CID 102316472.
Unlike a phosphoramidite, this reagent carries a 3'-O-succinate half-ester rather than a coupling group. The succinate is the conventional linker for attaching the 3'-terminal RNA nucleoside to an amino-functionalized solid support (such as controlled-pore glass), so that chain assembly starts from this residue. The succinyl linkage is base-cleavable and is broken during deprotection to release the finished oligonucleotide; 2'-O-TBDMS protects the 2'-hydroxyl, 5'-DMT protects the 5'-hydroxyl, and N6-benzoyl protects the adenine amine.
Synonyms
DMT-2'-O-TBDMS-rA(Bz)-3'-succinate TEA salt; 5'-O-DMT-N6-Bz-2'-O-TBDMS-adenosine-3'-O-succinyl triethylammonium salt
Identity and specifications
| Catalog No. | CH65117 |
| CAS No. | 131316-88-6 |
| Molecular Formula | C48H53N5O10Si (succinate) |
| Molecular Weight | 888.0 |
| PubChem CID | 102316472 |
| InChIKey | RGOXSXKJIIKVLC-WRPKRATBSA-N |
| Purity | 98% |
Storage conditions
-20 C; tightly closed; protected from humidity
Technical attributes
- Reagent class
- 3'-O-succinate support-loading precursor
- Terminal residue
- 2'-O-TBDMS-rA(Bz)
- Support linker
- Succinate half-ester
- Salt form
- Triethylammonium
- Packaging
- 100 mg; 250 mg; Custom packaging on request
- Physical form
- white to yellow powder
Application context
- 3'-terminal RNA support loading: the 3'-O-succinate couples to an amino-functionalized solid support to immobilize the 3'-terminal 2'-O-TBDMS-rA(Bz) residue.
- Base-cleavable linker: the succinyl ester is cleaved during deprotection, releasing the finished oligonucleotide from the support.
- Standard RNA protection: 2'-O-TBDMS protects the 2'-hydroxyl, 5'-DMT protects the 5'-hydroxyl, and N6-benzoyl protects the adenine amine.
Related technical resources
Oligonucleotide Modification
Sugar, base, backbone, terminal, and conjugation modifications for ASO, siRNA, guide RNA, aptamer, and research or labeling oligo projects.
Oligonucleotide Synthesis
Support for modified monomers, supports, sulfurizing reagents, cap analogs, and related oligo synthesis inputs.
Public references
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem Compound 102316472
PubChem · CID 102316472
Frequently Asked Questions
Is this a phosphoramidite?
No. It is a 2'-O-TBDMS RNA nucleoside 3'-O-succinate (a support-loading precursor), not a phosphoramidite. Its 3'-succinate half-ester attaches the 3'-terminal RNA residue to a solid support before chain assembly begins.
What is the succinate linker for, and is it cleaved?
The succinate couples the nucleoside to an amino-functionalized solid support (e.g. controlled-pore glass). It is a base-cleavable ester, so it is broken during deprotection to release the finished oligonucleotide.
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