3'-NH-Trt-2',3'-ddG (iBu) 5'-CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Product summary
Quick view
- Product family
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C42H51N8O5P
- MW
- 778.9
- Purity
- 99%
3'-NH-Trt-2',3'-ddG (iBu) 5'-CE Phosphoramidite is a building block for N3'→P5' phosphoramidate oligonucleotide chemistry: a 3'-tritylamino-2',3'-dideoxyguanosine with N2-isobutyryl protection and a 5'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite. Its PubChem-backed identity includes CAS 195375-66-7, molecular formula C42H51N8O5P, molecular weight 778.9, PubChem CID 135506969, and InChIKey USNNKXWWDUBLFP-MICLYWDSSA-N.
Unlike a standard amidite, the 3'-position carries a trityl-protected primary amine in place of the 3'-hydroxyl, and the phosphoramidite is on the 5'-position as a reverse amidite. During synthesis, the incoming monomer's 5'-phosphoramidite couples to the 3'-amino group of the growing chain, forming a P-N phosphoramidate bond (an N3'→P5' linkage) instead of the natural phosphodiester. Cited phosphoramidate literature reports nuclease-hydrolysis, duplex-stability, and RNA-like C3'-endo conformation context for this backbone class. N2-isobutyryl protects the guanine amine and is removed during final deprotection.
Synonyms
3'-amino-3'-deoxy-2'-deoxyguanosine(iBu) 5'-CE phosphoramidite, 3'-N-trityl; N3'->P5' phosphoramidate G building block
Identity and specifications
| Catalog No. | CH65126 |
| CAS No. | 195375-66-7 |
| Molecular Formula | C42H51N8O5P |
| Molecular Weight | 778.9 |
| PubChem CID | 135506969 |
| InChIKey | USNNKXWWDUBLFP-MICLYWDSSA-N |
| Purity | 99% |
Storage conditions
-20 C
Technical attributes
- Monomer class
- 3'-NH-Trt reverse CE phosphoramidite
- Nucleobase
- Guanine
- Base protection
- N2-isobutyryl
- Linkage role
- N3'-to-P5' phosphoramidate
- Grade
- N (Normal)
- Packaging
- 10 mg; 50 mg; 100 mg; 250 mg; 1 g; 5 g; 10 g; 25 g; 50 g; 100 g; 500 g; 1 kg; 5 kg; 20 kg; 25 kg; Custom packaging on request
- Physical form
- solid
- Stability
- powder: 3 years; in solvent: 1 year
Application context
- N3'→P5' phosphoramidate linkage: the 3'-amino group couples to the next residue's 5'-phosphorus, forming a P-N phosphoramidate bond in place of a phosphodiester.
- Reverse (5'-)amidite: the phosphoramidite is on the 5'-position and the 3'-amine is trityl-protected, so chain assembly runs in the 5'→3' sense for this chemistry.
- Backbone literature context: cited N3'→P5' phosphoramidate literature reports nuclease-hydrolysis, duplex-stability, and RNA-like C3'-endo conformation context; N2-isobutyryl protects the guanine amine.
Public references
- PubChem Compound 135506969
PubChem · CID 135506969
Frequently Asked Questions
What is an N3'→P5' phosphoramidate?
It is a modified backbone linkage in which the phosphorus joins the 3'-nitrogen of one nucleoside to the 5'-oxygen of the next (a P-N bond), instead of the natural 3'-oxygen phosphodiester. It is built from 3'-amino-3'-deoxy nucleosides like this one.
Why is the phosphoramidite on the 5'-position?
Because the 3'-position carries the amino group (trityl-protected) that becomes part of the phosphoramidate linkage, the coupling phosphoramidite is placed on the 5'-position — a 'reverse' amidite. Coupling joins the incoming 5'-phosphorus to the growing chain's 3'-amine.
What identifiers should match for CH65126?
Use CAS 195375-66-7, molecular formula C42H51N8O5P, molecular weight 778.9, PubChem CID 135506969, and InChIKey USNNKXWWDUBLFP-MICLYWDSSA-N.
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