LNA-A(Bz) CE Phosphoramidite
Oligonucleotide Synthesis, RNA Raw Materials & Modification
Produktübersicht
Kurzüberblick
- Produktfamilie
- Oligonucleotide Synthesis, RNA Raw Materials & Modification
- MF
- C48H52N7O8P
- MW
- 885.9
- Reinheit
- >=99% (HPLC)
LNA-A(Bz) CE Phosphoramidite is the locked nucleic acid (LNA) adenosine building block for oligonucleotide synthesis: a 2'-O,4'-C-methylene-bridged (locked) N6-benzoyladenosine with a 5'-O-(4,4'-dimethoxytrityl) group and a 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite group. CH65045 is identified by CAS 206055-79-0, molecular formula C48H52N7O8P, molecular weight 885.9, PubChem CID 13009021, and InChIKey HPIDKMAOZZZGOP-LIUGLABVSA-N.
The cited LNA literature discusses locked 2'-O,4'-C-methylene sugar structures in relation to oligonucleotide binding affinity and nuclease resistance. As a phosphoramidite, the 3'-phosphoramidite couples to the growing chain, the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection.
Synonyme
DMT-LNA-A(Bz) phosphoramidite; 5'-O-DMT-2'-O,4'-C-methylene-N6-benzoyladenosine 3'-CE phosphoramidite
Identität und Spezifikationen
| Catalog No. | CH65045 |
| CAS No. | 206055-79-0 |
| Molecular Formula | C48H52N7O8P |
| Molecular Weight | 885.9 |
| PubChem CID | 13009021 |
| InChIKey | HPIDKMAOZZZGOP-LIUGLABVSA-N |
| Purity | >=99% (HPLC) |
Lagerbedingungen
-20 C; protect from moisture and sunlight
Technische Merkmale
- Monomer class
- LNA CE phosphoramidite
- Nucleobase
- Adenine
- Sugar constraint
- 2'-O,4'-C-methylene bridge
- Base protection
- N6-benzoyl
- Grade
- N (Normal)
- Packaging
- 100 mg; 250 mg; 500 mg; 1 g; 5 g; 10 g; 25 g; 100 g; 200 g; Custom packaging on request
- Water content
- NMT 0.50%
- Physical form
- white to off-white solid; free from foreign particles
- Stability
- >=4 years
Anwendungskontext
- LNA coupling monomer: the 3'-O-(2-cyanoethyl N,N-diisopropyl)phosphoramidite is the reactive group that couples to the growing oligonucleotide chain in automated solid-phase synthesis.
- Locked (LNA) sugar: the cited LNA literature discusses the 2'-O,4'-C-methylene bridge in relation to oligonucleotide binding affinity and nuclease resistance.
- 5'-DMT + N6-benzoyl protection: the acid-labile 5'-DMT is removed each cycle, and the N6-benzoyl base group is removed at final deprotection.
Zugehörige technische Ressourcen
Öffentliche Quellen
- Sustainability Challenges and Opportunities in Oligonucleotide Manufacturing
Journal of Organic Chemistry, 2021
Product-family technical context
- Solid-phase supports for oligonucleotide synthesis
Current Protocols in Nucleic Acid Chemistry, 2013
Product-family technical context
- Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
Tetrahedron Letters, 1981
Product-family technical context
- Synthesis of deoxyoligonucleotides on a polymer support
Journal of the American Chemical Society, 1981
Product-family technical context
- PubChem-Verbindung 13009021
PubChem · CID 13009021
Häufig gestellte Fragen
What is LNA and what does this amidite do?
LNA (locked nucleic acid) contains a rigid 2'-O,4'-C-methylene bridge. This amidite's 3'-phosphoramidite couples to the growing chain, with 5'-DMT removed each cycle and N6-benzoyl removed at final deprotection.
How does LNA differ from 2'-OMe or 2'-F modifications?
They are different sugar-modification chemistries: LNA locks the sugar with a 2'-O,4'-C-methylene bridge, while 2'-OMe and 2'-F are 2'-substituted ribose/deoxyribose analogs. The 2'-OMe and 2'-F amidites are cataloged separately.
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